乳腺癌β-catenin表达与细胞凋亡关系研究  

On the Expression of β-catenin and Its Correlation with the Apoptosis of the Breast Carcinoma Cells

作　　者：杨剑锋[1] 陈森林[2] 刘志红[2] 高尼娜[2] 胡骏[2] 

机构地区：[1]湖南师范大学医学院,中国长沙410013 [2]湖南省肿瘤医院,中国长沙410013

出　　处：《湖南师范大学自然科学学报》2008年第3期115-117,共3页Journal of Natural Science of Hunan Normal University

基　　金：湖南省教育厅科研基金资助项目(04C354);卫生部科研基金资助项目(WKJ2007-3-001)

摘　　要：探讨β-catenin在乳腺癌组织中与细胞凋亡的关系.免疫组化SP法检测β-catenin、survivin在乳腺癌的表达,TUNEL检测细胞凋亡.乳腺癌组织中31例(68.9%)β-catenin表达异常,33例(73.3%)survivin阳性表达.β-catenin正常表达乳腺癌组织肿瘤细胞凋亡率为(9.89±2.58)%,β-catenin异常表达的肿瘤细胞凋亡率为(7.28±1.86)%,两组有显著性差异(t=3.86,P(0.01).在β-catenin正常表达病例中,42.9%(6/14)survivin表达阳性,而在β-catenin异常表达病例中,87.1%(27/31)survivin表达阳性(表1).β-catenin异常表达与survivin阳性表达有显著的正相关性(rs=0.463,P<0.01).β-catenin在浆、核内聚集的异常表达可能通过激活survivin的表达而抑制乳腺癌细胞的凋亡.To examine the expression of β-catenin in breast cancer and its correlation with the apoptosis. The expression of β-catenin, survivin in 45 cases of breast cancer was by using high sensitive SP immunohistochemical method. The apoptosis of breast carcinoma ceils was detected by TUNEL assay. The abnormal expression rates of β-catenin, survivin were 68.9% （ 31/45 ）, 73.3% （ 33/45 ） respectively. The apoptosis rate of cells （ 7.28 ± 1.86） % in cases with abnormal expression of β-catenin was significantly less than in normal cases （9. 89 ± 2. 58）%. 42. 9% （6/14） of the cases with normal staining of β-catenin showed positive expression of survivin, while 87.1% （27/31）of the cases showed positive expression of survivin with abnormal staining. Abnormal expression of β-catenin and positive expression of survivin showed a significantly positive correlation （rs= 0. 463, P 〈 0. 01 ）.β-catenin accumulation in cytoplasm/nuclear may inhibit the apoptosis of the breast carcinoma cells by triggering survivin expression.

关 键 词：Β-CATENIN SURVIVIN 凋亡 乳腺肿瘤 

分 类 号：O183[理学—数学]