萘降解菌N19-3的分离、鉴定和萘双加氧酶基因的检测  

Isolation and Characterization of Naphthalene-Degrading Bacteria Comamocas sp.N19-3 and Detection of Its Naphthalene-Dioxygenase Gene

作　　者：刘怡辰[1] 曹娟[1] 高国庆[1] 沈标[1] 

机构地区：[1]南京农业大学生命科学学院,农业部农业环境微生物工程重点开放实验室,江苏南京210095

出　　处：《环境科学研究》2008年第5期27-31,共5页Research of Environmental Sciences

基　　金：科学技术部农业微生物菌种资源整理整合及共享试点(2005DKA21201-11)

摘　　要：从石油污染土壤中分离到一株高效降解萘的N19-3菌株.经形态观察、生理生化实验和16S rDNA序列分析等鉴定其为丛毛单胞菌属（Comamonassp.）.该菌株能在30℃,30 h内将1 000 mg/L的萘完全降解.降解萘的适宜温度为20-30℃,适宜pH为7.0-9.0.0.1 mmol/L的Ca^2＋和Fe^3＋对N19^-3菌株降解萘有较强的促进作用,0.1 mmol/L的Mn^2＋和Zn^2＋对N19-3菌株的生长和萘的降解也有一定的促进作用,而0.1 mmol/L的Cu2＋则完全抑制了N19-3菌株的生长和萘的降解.通过PCR方法在N19-3菌株中扩增出分别与C.testosteroniH菌株的萘双加氧酶铁硫蛋白大亚基基因（pahAc）与双加氧酶铁硫蛋白小亚基基因（pahAd）高度同源的核苷酸片断.A naphthalene-degrading bacterium （referred as N19-3） was isolated from the oil-contaminated soil. Based on morphological, physiological and biochemical characteristics and 16S rDNA sequences, N19-3 was identified as Comamocas sp. Strain N19-3 could degrade 1 000 mg/L naphthalene completely within 30 hour at 30 ℃ . The optimum temperature and pH for growth and naphthalene degradation were 20 - 30 ℃ and pH 7.0 - 9.0 respectively. The Ca^2 , Fe^3＋ （0.1 mmol/L） could greatly enhance the growth of strain N19-3 and degradation of naphthalene. The Mn^2＋ and Zn^2＋ （0.1 mmol/L） also promoted the growth of strain N19-3 and degradation of naphthalene. But the Cu^2＋ （0.1 mmol/L） could absolutely inhibit the growth and degradation of naphthalene. Two genes were obtained which were highly homologous to pahAc and pahAd genes encoded the large and small subunit of naphthalene dioxygenase from C. testosteroni H by PCR.

关 键 词：丛毛单胞菌属 分离鉴定 萘双加氧酶基因 

分 类 号：X703.1[环境科学与工程—环境工程]