牙鲆GHR基因Promoter区微卫星序列多态性与生长性状关系的初步研究  

Microsatellite Polymorphism Analysis of GHR Gene Promoter Region in Paralichthys olivaceus Hatchery Stocks and Its Association with Growth Traits

作　　者：倪静[1,2] 尤锋[1] 于深辉[1,2] 张培军[1] 徐冬冬[1,2] 吴志昊[1,2] 文爱韵[1,2] 徐永立[1] 

机构地区：[1]中国科学院海洋研究所,山东青岛266071 [2]中国科学院研究生院,北京100039

出　　处：《中国海洋大学学报（自然科学版）》2008年第5期719-725,共7页Periodical of Ocean University of China

基　　金：国家高技术研究发展规划项目(2006AA10A404);国家自然科学基金(30571445);国家科技基础条件平台工作项目-水产种质资源标准化整理、整合与共享课题(2004DKA30470-017)资助

摘　　要：采用牙鲆GHR基因5’端Promoter区的1个微卫星标记,对胶南和日照2个牙鲆养殖群体进行了群体遗传多样性的研究,并探索该基因多态性位点与牙鲆生长性状之间的相关性。结果表明,2个群体在该座位的等位基因数为12和9个,有效等位基因数为6.26和5.04个,多态信息含量为0.84和0.80。2个群体该座位的Hardy-Weinberg遗传偏离指数均为正值,并没有显示出杂合子缺失,但各基因型分布频率都在一定程度上偏离Hardy-Weinberg平衡(P<0.01)。连锁分析中发现,在胶南群体中,IM基因型对应的个体在全重、全长、体长、头长、体高和眼径形态学数据中均是最大的;在日照群体中,BC基因型对应的个体在全重、全长、体高、尾柄高、尾柄长和眼径数据中均是最大的;而CJ基因型对应的个体在体长和头长这两组数据中是最大的。该结果为研究GHR基因的变异对鱼类生长发育的影响及探索将该基因作为生长遗传标记的可行性奠定了实验基础。Genetic diversity of two cultured stocks of the left eyed flounder （Paralichthys olivaceus） from Jiaonan Country and Rizhao Country were analysed by using one microsatellite loci in the 5＇ promoter region of growth hormone receptor（ GHR ） gene, and the polymorphisms and their associations with growth traits were also investigated. The results showed that the allele numbers were 12 and 9, respectively, the effective alleles numbers were 6.26 and 5.04, the Fixation Indexes were-0.02 and-0.20, the Shannon indexes were 2.08 and 1.82, the heterozygosity observed values were 0.96 and 0.85, the unbiased expected heterozygosity values were 0.84 and 0.81, the values of polymorphism information content were 0.84 and 0.80 in the two stocks. The deviations from Hardy-Weinherg index of the two stocks were both positive, which did not show lack of heterozygosity. While the chi-square test presented that this microsatellite locus in both stocks were deviated from the Hardy-Weinberg equiiibrium（P 〈 0.01）. SPSS was used to study the possible association of the detected variations with growth traits. The evaluation of association effects between these microsatellite patterns with weight, total length, body length, head length, body height and distance between eye suggests the individuals with genotype IM was the highest in the Jiaonan stock（P 〈 0.05）. While in the Rizhao stock, values of weight, total length, body height, tail height, tail length and distance between eyes of the individuals with genotype BC were higher than those of the other genotypes, and values of body length and head length of the individuals with genotype CJ were higher than those of the other genotypes. These data would provide a potential use for GHR gene as the genetic marker of fish growth in the future MAS.

关 键 词：牙鲆 GHR基因5’端Promoter区 微卫星 遗传多样性 性状连锁分析 

分 类 号：S917[农业科学—水产科学] Q953[生物学—动物学]