肾络通对阿霉素诱导的肾病大鼠TGF-β_1、TGF-β_1 mRNA及c-fos表达的影响  

Effect of Shengluotong on Expression of TGF-β_1,TGF-β_1 mRNA and c-fos in Rats with Adriamycin-induced Nephrothy

作　　者：丁跃玲[1] 赵玉庸[2] 丁英钧[2] 

机构地区：[1]河北医科大学中医学院,石家庄050091 [2]河北医科大学中西医结合学院

出　　处：《河北中医药学报》2008年第3期3-4,14,共3页Journal of Hebei Traditional Chinese Medicine and Pharmacology

基　　金：河北省卫生厅资助项目:No 2003057

摘　　要：目的:观察中药肾络通对肾小管-间质纤维化大鼠TGF-β1、TGF-β1 mRNA及c-fos基因表达的影响。方法:建立单侧肾切除加重复阿霉素注射所诱导的肾病模型,采用病理形态学、免疫组化、原位杂交、流式细胞仪检测等方法,观察TGF-β1、TGF-β1 mRNA及c-fos基因表达特点及肾络通的影响。结果:模型组呈强阳性表达,肾络通组较模型组明显减弱。结论:活血化瘀中药肾络通能够抑制TGF-β1、TGF-β1 mRNA及c-fos基因表达,减轻肾小管间质纤维化,减缓慢性肾功能衰竭的进展。Objective:to observe the effect of Shenluotong on the expression of TGF-β1,TGF-β1mRNA and c-fos in rats with renal tubule interstitial fibrosis. Methods: The model group was made by unilateral nephrectomy with twiceamycin injections by caudal vein.Immunohistochemical method,in situ hybridization,flow cytometry and pathologic image pattern analysiswere used in the test to observe the expression of TGF-β1,TGF-β1mRNA and c-fos in renal tissue and the effect of Shenluotong.Results: it had over-expression in model group,and it expressed slightly in Shenluotong group.Conclusion: Shenluotong could inhibit the expression of TGF-β1,TGF-β1mRNA and c-fos,alleviate renal tubule interstitial fibrosis,and prolong the progress of CRF.

关 键 词：肾络通 肾小管-间质纤维化 转化生长因子-β1 MRNA c-fos 流式细胞分析 定量分析 

分 类 号：R285.5[医药卫生—中药学]