编码HIV-1 Pr42^(gag)的杆状病毒转移载体的构建及鉴定  

Construction and Identification of Recombinant Baculovirus Transfer Vector Encoding Human Immunodeficiency Virus Type 1 Pr42 gag

作　　者：张富泉[1,2] 张红毅[1,2] 马文煜[1,2] 姜绍谆 樊英茹[1,2] 任君萍 

机构地区：[1]第四军医大学微生物学教研室 [2]英国伦敦CharingCross医院生化室

出　　处：《细胞与分子免疫学杂志》1997年第3期14-17,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金

摘　　要：本文作者采用聚合酶链式反应（ＰＣＲ），从ＨＩＶ－１ｃＤＮＡ克隆ＢＨ１０中扩增出Ｐｒ４２ｇａｇ的基因片段，经适当的限制性内切酶消化后插入到杆状病毒转移载体ｐＢａｃＰＡＫ９中，使其处于多角蛋白启动子的控制之下，构成重组转移载体ｐＢａｃＧＡＧ４２。酶切鉴定结果与预期的一致。再用载体上多克隆位点两翼的引物对连接处进行序列分析，结果表明外源基因处于多角蛋白启动子的控制下，且成功地引入了起始码和终止码。We amplified the gene of human immunodeficiency virus type 1(HIV)Pr42 gag by polymerase chain reaction(PCR) from the HIV cDNA clone BH10, and inserted it into the baculovirus transfer vector pBacPAK9 The recombinant transfer vector,designated as pBacGAG42, was characterized by restriction endonuclease digestion and sequence analysis with a primer pair flanking the multiple cloning sites The results showed that the target gene was inserted properly, and under the control of baculovirus polyhedrin promoter, in addition, in frame translation initial codon (ATG) and stop codon(TAG) were successfully incorporated by primer mismatching

关 键 词：基因重组 杆状病毒 转移载体 艾滋病毒疫苗 

分 类 号：R392－33[医药卫生—免疫学] Q782[医药卫生—基础医学]