从HCV蛋白一级结构预测其C,E,NS5区的CD_4^+ T细胞抗原位点  

Prediction of CD 4 + T Cell Antigen Sites in C, E, and NS5 Regions from the Primary Structure of HCV Proteins

作　　者：周红超[1,2] 徐德忠[1,2] 张鹏 闫永平[1,2] 张景霞 李远贵[1,2] 杜尊宪 李玲秀[1,2] 梁国政 

机构地区：[1]第四军医大学流行病学教研室 [2]西安市传染病院

出　　处：《细胞与分子免疫学杂志》1997年第3期18-22,13,共6页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金

摘　　要：我们根据ＣＤ４＋Ｔ细胞识别抗原位点的物理化学和生物学特征，设计了一个具有查找两亲性螺旋状结构（ａｍｐｈｉｐａｔｈｉｃｈｅｌｉｘｓｔｒｕｃｔｕｒｅ）肽段功能的计算机程序。用该程序对ＨＣＶ－１型病毒Ｃ、Ｅ（Ｅ１、Ｅ２／ＮＳ１）、ＮＳ５蛋白一级结构进行分析，发现这些蛋白区存在ＣＤ４＋Ｔ细胞识别位点。此结果支持了ＣＤ４＋Ｔ细胞对ＨＣＶＣ，Ｅ，ＮＳ５区可发生增殖反应的结论。提示该程序可作为一种预测ＣＤ４＋Ｔ细胞识别ＨＣＶ抗原位点的方法。A computer programme with the function of finding amphipathic helix structure was written on the basis of physical, chemical and biological characters of antigen sites that can stimulate CD 4 + T cell.Analysis of the hepatitis C virus (HCV 1) primary sequence of C, E, and NS5 regions showed that the CD 4 + T cell antigen sites could be found in these regions. The findings were in agreement with the conclusion that C, E and NS5 proteins could activate CD 4 + T cell in HCV patients. It indicates that our programme can be used to predict the CD 4 + T cell antigen sites on HCV proteins.

关 键 词：CD4^+T细胞 丙型肝为病毒 抗原位点 预测 

分 类 号：R373.2[医药卫生—病原生物学] R512.630.3[医药卫生—基础医学]