中国广东地区汉族补体C4遗传多态性分析  

Polymorphism of Complement C4 of Han Nationality Chinese in Guangdong Area

作　　者：张明军[1,2] 陈仁馨[1,2] 黄为民 刘新[1,2] 

机构地区：[1]第一军医大学免疫学教研室 [2]珠江医院

出　　处：《细胞与分子免疫学杂志》1997年第3期31-34,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：广东省科学技术委员会科学基金

摘　　要：采用国际补体参考实验室方法，分析了广东地区３１９例无血缘关系汉族人Ｃ４多态性。共检出５个Ｃ４Ａ和８个Ｃ４Ｂ同种异型：Ｃ４Ａ５、４、３、２及Ｑ０和Ｃ４Ｂ５、４、２、１、９１、９６、９５及Ｑ０。Ｃ４Ａ以Ａ＊３频率（０．６５３８）最高，Ａ＊４（０．１６３５）、Ａ＊Ｑ０（０．１３９４）及Ａ＊２（０．０４１７）次之。Ｃ４Ｂ以Ｂ＊２（０．４４０７）和Ｂ＊１（０．４１０３）最高，Ｂ＊Ｑ０（０．０９４６）、Ｂ＊４（０．０２４０）及Ｂ＊５（０．０１６０）次之。其余Ｃ４同种异型少见（基因频率均＜０．０１）。杂合Ｃ４ＡＱ０和杂合Ｃ４ＢＱ０表型个体占总体的２３．５１％和１４．１１％，纯合Ｃ４ＡＱ０（Ｃ４ＡＱ０，Ｑ０）和纯合Ｃ４ＢＱ０（Ｃ４ＢＱ０，Ｑ０）均占２．５１％，纯合Ｃ４ＡＱ０、ＢＱ０（Ｃ４ＡＱ０，Ｑ０ＢＱ０，Ｑ０）占０．９４％。此外，发现６例Ｃ４Ｂ重复位点。经Ｈａｒｄｙ－Ｗｅｉｎｂｅｒｇ卡方检验，Ｃ４Ａ和Ｃ４Ｂ基因频率分布达到遗传平衡。The C4 genetic polymorphism of 319 healthy Han nationality Chinese without any blood relationship in Guangdong area was detected and analyzed with the methods adopted from the international complement reference laboratory. Five C4A(5, 4, 3, 2, Q0) and eight C4B(5, 4, 2, 1, 91, 96, 95, Q0) allotypes were obtained. The highest frequency genes were C4A *3(0.6538) for C4A; C4B *2(0.4407) and C4B *1(0.4103) for C4B. The less common ones were C4A *4(0 1635), A *Q0(0 1394) and A *2(0.0417) for C4A; C4B *Q0(0 0946), B *4(0 0240) and B *5(0 0160) for C4B. The others were rare (gene frequency <0.01). Heterozygous C4AQ0 and heterozygous C4BQ0 individuals were estimated as 23.51% and 14.11% respectively. Homozygous C4AQ0(C4AQ0, Q0) and homozygous C4B(C4BQ0, Q0) both accounted for 2 51%. Homozygous C4AQ0, BQ0(C4AQ0, Q0BQ0, Q0) with a percentage of 0 94% was found. Six C4B duplication loci were also found. The distributions of C4A and C4B gene frequencies were in accordance with those expected from the Hardy Weinberg equilibrium by χ 2 test.

关 键 词：补体 C4 遗传多态性 汉族 中国 广东 

分 类 号：Q346.5[生物学—遗传学] Q986