抗人心肌肌凝蛋白轻链单克隆抗体的制备和鉴定  

Preparation and Identification of Monoclonal Antibodies against Human Cardiac Myosin Light Chain 1

作　　者：刘家慧[1] 张一泉[1] 褚丽萍[1] 张桂华[1] 马春节[1] 张晓蓉 林汉[1] 

机构地区：[1]中国医学科学院中国协和医科大学放射医学研究所

出　　处：《细胞与分子免疫学杂志》1997年第3期42-44,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：天津市自然基金;中国医学科学院基金

摘　　要：从人心脏中提取心肌肌凝蛋白轻链（ＣＭＬＣ１），免疫ＢＡＬＢ／ｃ小鼠，取其脾细胞与Ｓｐ２／０骨髓瘤细胞做常规融合，获得了２２株分泌抗ＣＭＬＣ１单克隆抗体的杂交瘤细胞株。经对其中某些株作ＩｇＧ分型、核型、腹水效价、表位和亲和常数测定，表明所获单克隆抗体的质量良好，为建立分析轻链的方法奠定了基础。BALB/c mice were immunized with cardiac myosin light chain 1(CMLC 1) purified from human heart The spleen lymphocytes were got and fused with Sp2/0 mouse myeloma cells by employing routine procedure Twenty two hybridoma cell lines secreting monoclonal antibodies specific for CMLC 1 were obtained The IgG isotypes, titer, epitopes and affinity constant of some monoclonal antibodies were determined and chromosomes of some hybridoma cells were observed The results showed that some monoclonal antibodies achieved high qualities and were suitable for establishment of assay method of myosin light chain in sera further

关 键 词：单克隆抗体 肌凝蛋白 轻链 CMLC1 制备 

分 类 号：R392－33[医药卫生—免疫学]