缺铁性贫血大鼠铁效应元件结合蛋白mRNA表达及顺乌头酸酶活性测定  

Expression of iron responsive element binding protein mRNA and analysis of aconitase activity in iron deficiency anemia rats

作　　者：李昕权[1,2] 李丰益[1,2] 廖清奎[1,2] 罗春华 

机构地区：[1]华西医科大学附属第二医院 [2]军事医学科学院附属医院

出　　处：《中华血液学杂志》1997年第11期563-565,共3页Chinese Journal of Hematology

基　　金：中国博士后科学基金

摘　　要：目的：探索铁代谢平衡中铁效应元件结合蛋白（ＩＲＥ－ＢＰ）的调节机制。方法：用低铁饮食建立大鼠营养性缺铁性贫血模型，分析缺铁性贫血发生过程中ＩＲＥ－ＢＰｍＲＮＡ的表达水平及顺乌头酸酶活性的变化。结果：ＩＲＥ－ＢＰｍＲＮＡ表达水平在缺铁性贫血发生、发展过程中无明显变化，而顺乌头酸酶活性明显降低，再经硫酸亚铁处理后活性明显升高。结论：ＩＲＥ－ＢＰ可能通过其内部［Ｆｅ－Ｓ］基团变构的翻译后调节与转录后调节共同参与铁代谢平衡的调控。Objective:To explore the regulatory mechanism of iron responsive element binding protein(IREBP) in iron metabolism.Methods:The expression level of IREBP mRNA and the aconitase activity were studied in iron deficiency anemia (IDA) rat model.Results:No distinct change of IREBP mRNA expression was observed in iron deficiency rats.The aconitase activity of liver cytoplasmic protein was much lower in iron deficiency rats than that in controls and treatment of liver cytoplasmic protein with FeSO4 resulted in a strong stimulation of the enzymatic activity.Conclusion:Both posttranslational and posttranscriptional regulation of IREBP may take part in iron metabolism balance.

关 键 词：缺铁性贫血 结合蛋白 mPNA 乌头酸酶 测定 

分 类 号：R556.3[医药卫生—血液循环系统疾病]