参芎注射液对大鼠脑缺血再灌注后神经细胞凋亡的相关研究  

The relative study on the effect of Shenxiong injection on the neuronal apoptosis in rat after cerebral ischemia-reperfusion

作　　者：张智博 陈本阳[2] 唐璇[2] 彭旭[2] 唐璐[2] 

机构地区：[1]长沙市第一医院神经内科,长沙410005 [2]南华大学内科教研室

出　　处：《中华老年心脑血管病杂志》2008年第10期775-778,共4页Chinese Journal of Geriatric Heart,Brain and Vessel Diseases

摘　　要：目的探讨参芎注射液对大鼠脑缺血再灌注后神经细胞凋亡及凋亡相关因子caspase-8、抗凋亡因子FLIP的蛋白及其mRNA表达的影响。方法选择SD大鼠100只随机分为正常组(10只)、假手术组(10只)、模型组(40只)和参芎注射液治疗组(干预组,40只),采用TUNEL法检测神经细胞凋亡,免疫组织化学及RT-PCR法分别检测caspase-8、FLIP的蛋白和mRNA表达。结果与正常组和假手术组比较,模型组大鼠各时间点凋亡细胞明显增加(P<0.01),caspase-8、FLIP的蛋白和mRNA表达明显增强(P<0.05,P<0.01)。与同时间点模型组比较,干预组大鼠凋亡细胞明显减少,caspase-8蛋白和mRNA表达明显减弱(P<0.05,P<0.01),FLIP蛋白和mRNA表达分别于再灌注6 h和12 h时间点明显增强(P<0.05)。结论参芎注射液能通过减弱caspase-8表达及增强FLIP表达,从而拮抗大鼠脑缺血再灌注后神经细胞凋亡。Objective To study the effect of Shenxiong injection on the neuronal apoptosis and the expression of the protein and mRNA of caspase 8 and FLIP in rat after cerebral ischemia-reperfusion. Methods 100 healthy male SD rats were randomly divided into 4 groups：normal group（n= 10）, sham operated group（n= 10） ,model group（n=40） ,Shenxiong injection treated group （trea ted group,n=40）. A model of cerebral middle artery occlusion in rat was performed with intraluminal filament occlusion. The neuronal apoptosis was detected by the method of TUNEL,and the expression of the protein and mRNA of caspase-8 and FLIP was respectively evaluated by meth- ods of immunohistoehemistry and RT-PCR. Results Compared with normal group and sham-operated group,the apoptotic cells and the expression levels of the protein and mRNA of caspase-8 and FLIP in the rats of model group increased significantly （P〈0.05, P〈0.01）. Compared with model group,the apoptotic cells and the expression levels of the protein and mRNA of caspase-8 in the rats of treated group decreased significantly （P〈0.05 ,P〈0.01）, the expression levels of the protein and mRNA of FLIP in them increased significantly at 12 h and 6 h （P〈0.05）. Conclusion Shenxiong injection can decrease the expression levels of caspase-8 protein and mRNA, and increase the expression levels of FLIP protein and mRNA in rat after cerebral ischemia-reperfusion,thus inhibiting the neuronal apoptosis.

关 键 词：脑缺血 再灌注 细胞凋亡 参芎注射液 半胱氨酸天冬氨酸蛋白酶 

分 类 号：R285.5[医药卫生—中药学]