一种建立门脉高压动物模型的新方法  

A new method for setting up animal model of portal hypertension

作　　者：钱骏[1] 冯敢生[1] 梁惠民[1] 杜端明[1] 郑传胜[1] 

机构地区：[1]同济医科大学附属协和医院放射科,武汉市430022

出　　处：《放射学实践》1997年第4期141-144,共4页Radiologic Practice

基　　金：国家自然科学基金!No:39570232

摘　　要：目的：探索一条新的、更迅速和有效地建立门脉高压和肝纤维化动物模型的途径。材料和方法：采取前瞻性研究方法，在15只大耳白兔通过门静脉主干注入不同稀释度的白芨混悬液，栓塞门静脉末梢血管而升高门脉压，术后3～4周采取同法复查测量门脉压，作肝活检病理切片及血管铸形。结果：通过兔门静脉注入0．4％白芨混悬液4ml，能使门脉压升高约40％，3周后门脉压仍维持较高水平，病理切片示被白芨栓塞部位出现典型的肝纤维化改变，血管铸形见被白芨栓塞的末梢血管约占全肝末梢血管容积的20％．结论：本法操作简便，效果理想，重复性好，药物价格低廉，较其它建立门脉高压动物模型的方法更优越和可靠，有着广泛应用的价值。To explore a new, rapid and effective way to establish a portal hypertension and liver cirrho-sis animal model. Materials and methods:By using prospective investigating method,various dilute sus-pensions of Bletilla striata were intravenously injected to embolise peripheral portal vein for increasing portal vein pressure in 15 rabbits.3～4 weeks and 6 ～7 weeks after operation, portal vein pressure was deter-mined again; liver tissue biopsy and microscopy were examined; vascular casting was made .Results: Portalvein pressure increased by 40% after intraenously administration of 0.4% Bletilla striata 4ml into portalvein. 3 weeks aller operation, it was showed tha the portal vein pressure was still in high level; typical livercirrhosis change change was observed in the areas of embolism by Bletilla striat in microscopy; the embolised pe-ripheral blood vessels occupied about 20% of total peripheral blood vessels of the liver in vascular casting.Conclusion:The methed descrital ahae showed tha it is easy to opend, ideal,reproducible; the price ofBletilla striata is cheap.The method is also reliable and better than others,it has wide useful value

关 键 词：门脉高血压 动物模型 

分 类 号：R657.34[医药卫生—外科学]