家兔面神经撞击伤后生长相关蛋白的变化及丙酸睾丸酮治疗的影响  

Effect of Testosterone Propionate on Changes of Growth Associated Protein in Facial Nucleus After Right Facial Nerves Impacted in Rabbits

作　　者：姜燕平[1] 王正国[1] 朱佩芳[1] 刘宝松[1] 周元国[1] 王国强[1] 

机构地区：[1]第三军医大学野战外科研究所四室

出　　处：《中华创伤杂志》1997年第6期360-362,共3页Chinese Journal of Trauma

摘　　要：目的：研究面神经撞击伤后面神经核内生长相关蛋白（ＧＡＰ－４３）的变化及丙酸睾丸酮（ＴＰ）治疗的影响．方法：利用ＢＩＭ－Ⅱ型生物撞击机致家兔右侧面神经干损伤，ＳＰ免疫组化染色法观察损伤后１、３、７、１４和２１天伤侧面神经核内ＧＡＰ－４３的改变，电镜观察面神经干超微结构变化，以及ＴＰ对这些改变的影响．结果：面神经损伤第１天，伤侧面神经核内ＧＡＰ－４３样免疫反应性神经元（ＧＬＩＮ）开始增多，第１４天达到高峰，第２１天逐渐下降．ＴＰ治疗组各时相点ＧＬＩＮ的增多明显高于对照组．与对照组相比，伤后第２１天ＴＰ组面神经干电镜观察可见较多的新生髓鞘．结论：ＴＰ治疗能促进伤后面神经核内ＧＬＩＮ的合成。Aim: To study the effect of testosterone propionate (TP) on changes of a growth associated protein (GAP43) in facial nucleus after right facial nerves impacted in rabbits. Methods: Facial nerve impacted injury model was made with type BIMⅡ biologic impact machine in the right facial nerves of New Zealand rabbits. Both changes of GAP43 such as immunohistochemistic neurons (GLIN) in the right facial nerve nucleus and the ultrastructure of facial nerve on 1st, 3rd, 7th, 14th and 21st day after injury were observed with streptavidinperoxidase (SP) immunohistochemistry staining and with electron microscopy respectively. Results: GLIN in the facial nerve nucleus in the damaged side began to increase at the 1st day and reached peak at the 14th day of injury, and to decrease gradually at the day of 21st. GLIN in TPtreated group increased significantly compared with that of the control group. There was more new myelinogeny in facial nerves in the TPtreated group observed under electronic microscope on the 14th day after injury when compared with that of the controls. Conclusion: TP may help the injured facial nerves in repairing and regenerating by increasing the synthesis and the expression of GAP43.

关 键 词：面神经损伤 生长相关蛋白 丙酸睾丸酮 治疗 

分 类 号：R651.305[医药卫生—外科学]