SD大鼠骨髓基质细胞体外培养及分化研究  

The study on cultivation and differentiation of marrow stromal cells of SD rats in vitro

作　　者：石红光[1] 吕渭莉[1] 黄磊[1] 

机构地区：[1]广州市第一人民医院口腔科,广东广州510180

出　　处：《国际医药卫生导报》2008年第20期13-15,12,共4页International Medicine and Health Guidance News

基　　金：广州市医药卫生科技项目（编号：2006-YB-044）

摘　　要：目的研究SD大鼠骨髓基质细胞（marrow stromal cells，MSCs）的生物学特性及其成骨和成脂分化能力。方法处死16只6月龄SD大鼠动物，非连续密度梯度离心分离大鼠MSCS，传代后分别在成骨及成脂培养基中诱导培养14天，观察细胞形态，绘制细胞生长曲线，通过组织特染对MSCs的成骨、成脂表型进行鉴定，计数阳性染色细胞百分比。结果SD大鼠MSCS在体外具有成骨细胞分化潜力，油红0染色显示成脂诱导培养基可定向诱导其分化。结论本实验成功分离SD大鼠MSCs且保持分化能力。Objective To study the biological Characteristics and osteoblast/adipocyte phenotypes of bone marrow stromal cells （BMSCs）of Sprague-Dawley（SD）rats. Methods 16 SD rats with Six-month-old were executed in this study. BMSCs of 16 rats were isolated by discontinueous gradient centrifugation and then plated in a -MEM medium as primary culture. Secondary harvested cells were induced cultured for 14 days in the osteogenic medium or the adipogenic medium. Observe the ceils＇ morpha, The cells were screened by inverted microscope each day and cell growth was studied with cell counting. The osteoblast and adipocyte phenotypes were verified by cytochemistry staining, counted the percentage of positive stained cells. Results Gomori and Von Kossa＇ s staining demonstrated positive osteoblast phenotypes of alkaline phosphatase and mineralized nods by osteogenic inducer, while Oil Red 0 staining identified BMSCs treated with adipogenic medium resulted in adipocyte formation. Conclusion BMSCs of SD rats has been isolated successfully and maintain their differentiation potential.

关 键 词：骨髓基质细胞 成体干细胞 成脂肪表型 

分 类 号：R3[医药卫生—基础医学]