甲基叔丁醚及其代谢产物对HL-60细胞的遗传毒性测定  

Cytotoxicity and Genotoxicity of Methyl Tertbutyl Ether and Its Metabolite to Human Leukemia Cells

作　　者：唐国慧[1,2,3] 王俊南 庄志雄 

机构地区：[1]西安医科大学卫生系 [2]新加坡国立大学社区职业家庭医学系 [3]中山医科大学公共卫生学院

出　　处：《中华预防医学杂志》1997年第6期334-337,共4页Chinese Journal of Preventive Medicine

基　　金：美国中华医学基金会(CMB)提供资助

摘　　要：应用单细胞凝胶电泳法（ＳＣＧＥ）测定新型汽油添加剂甲基叔丁醚（ＭＴＢＥ）及其代谢产物叔丁醇（ＴＢＡ）、α－羟基异丁酸（ＨＩＢＡ）和甲醛引起的人白血病细胞（ＨＬ－６０细胞）ＤＮＡ损伤，以乳酸脱氢酶的释放评价其细胞毒性。结果显示，ＭＴＢＥ、ＴＢＡ和ＨＩＢＡ在１～３０ｍｍｏｌ／Ｌ浓度范围内能够引起ＤＮＡ损伤并有明显的剂量反应关系；另一代谢产物甲醛在浓度５μｍｏｌ／Ｌ时即可产生ＤＮＡ损伤，但浓度升高时出现ＤＮＡ迁移减少。提示ＭＴＢＥ及其代谢产物可能具有遗传毒性。然而在引起ＤＮＡ损伤的剂量范围内却没有观察到ＭＴＢＥ、ＴＢＡ和ＨＩＢＡ的细胞毒性。DNA damage of human leukemia (HL60) cells caused by methyl tertbutyl ether (MTBE), a new gasoline additive, and its metabolites tertbutyl alcohol (TBA), αhydroxyisobutyric acid (HIBA) and formaldehyde was determined by single cell gel electrophoresis (SCGE), with release of lactate dehydrogenase as an indicator for evaluating its cytotoxicity. Results showed that MTBE, TBA and HUBA at levels of 1 to 30 mmol/L could cause DNA damage in a dosedependent pattern. Formaldehyde at level of 5 μmol/L could cause DNA damage, but at a higher level could decrease DNA migration. It suggested that MTBE and its metabolites could have genotoxicity, however, with doses causing genotoxic effects, no cytotoxic effect by MTBE, TBA and HIBA was observed, but formaldehyde presented obvious cytotoxic effect.

关 键 词：甲基叔丁醚 诱变力试验 遗传毒性 HL-60细胞 

分 类 号：R996[医药卫生—毒理学]