b-DAZL:A novel gene in bovine spermatogenesis  

作　　者：Qingbo Zhang Qifa Li Jiahuang Li Xinfu Li Zhenshan Liu Dawei Song Zhuang Xie 

机构地区：[1]Laboratory of Animal Reproductive Genetics and Molecular Evolution, College of Animal Science and Technology, Nanjing Agriculture University, Nanjing 210095, China [2]Pharmaceutic Biotechnology Key Laboratory, College of Life Science, Nanjing University, Nanjing 210093, China

出　　处：《Progress in Natural Science:Materials International》2008年第10期1209-1218,共10页自然科学进展·国际材料（英文版）

基　　金：supported by National Natural Science Foundation of China (Grant No.30500360);Youth Science and Technology Innovation Fund for young scientists of Nanjing Agriculture University (KJ05011)

摘　　要：The DAZL gene plays an important role in gametogenesis. Microdeletion and mutation of this gene has been frequently associated with the sterility in both vertebrates and invertebrates. Here, a cDNA containing the complete open reading frame of DAZL gene, which encodes putative RRM RNA-binding protein, was isolated from bovine tissues by RT-PCR. The isolated gene was referred to as b-DAZL. Computer-based location analysis showed that b-DAZL is located near the end of chromosome 1. The b-DAZL gene and its vertebrate homologue shared a high nucleotide similarity （90-94%） in the coding region and in the genomic organization, each consisting of 10 exons and 9 introns. Their protein products contained a highly conserved RNA-binding motif, a unique DAZ repeat, and PUM2 and PABP interacting domains. RT-PCR assay revealed that b-DAZL was expressed in the testis and ovary. However, no signals were detected in the testes of sterile male cattle-yaks. The microstructure of the testes of sterile males showed very few spermatocytes but mostly somatic cells. These observations are consistent with the typical phenotype of testes with defective DAZL expression. Subsequent gene detection studies indicated that transcription arrest might lead to a b-DAZL transcript defect in testes of cattle yaks. Our results suggest that b-DAZL may be involved in spermatogenesis, and that transcription arrest of the gene is associated with male sterility in cattle-yaks.DAZL 基因在 gametogenesis 起一个重要作用。这基因的 Microdeletion 和变化经常在脊椎动物和无脊椎动物与绝育被联系了。这里，包含 DAZL 基因的完全的开的读物框架的 cDNA，它编码通常认为的 RRM RNA 有约束力的蛋白质，被 RT-PCR 从牛的纸巾孤立。孤立的基因被叫作 b-DAZL。基于计算机的地点分析证明 b-DAZL 在染色体 1 的结束附近被定位。b-DAZL 基因和它的脊椎动物相当或相同事物分享了高核苷酸类似(90 94%) 在编码区域并且在 genomic 组织，每由 10 前 ons 和 9 introns 组成。他们的蛋白质产品包含了一个高度保存的 RNA 有约束力的主题，一次唯一的 DAZ 重复，和 PUM2 和 PABP 交往领域。RT-PCR 试金表明 b-DAZL 在睾丸和卵巢被表示。然而，没有信号在无菌的雄的牛牦牛的睾丸被检测。不育的男性的睾丸的微观结构显示出很少的 spermatocytes 但是主要体的房间。这些观察与有有缺点的 DAZL 表示的睾丸的典型显型一致。随后的基因察觉研究显示那抄写拘捕可能在牛牦牛的睾丸导致一个 b-DAZL 抄本缺点。我们的结果建议 b-DAZL 可以涉及精子发生，并且基因的那抄写拘捕在牛牦牛与男绝育被联系。

关 键 词：CATTLE-YAK b-DAZL Male sterility Sequence analysis Expression profile 

分 类 号：S823[农业科学—畜牧学]