Cloning and characterization of two putative seven-transmembrane receptor genes from cotton  

作　　者：Peng Gao Piming Zhao Juan Wang Haiyun Wang Guiling Wang Guixian Xia 

机构地区：[1]National Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Datun Road, Chaoyang District, Beijing 100101, China National Center for Plant Gene Research, Beijing 100101, China

出　　处：《Progress in Natural Science:Materials International》2008年第10期1225-1231,共7页自然科学进展·国际材料（英文版）

基　　金：supported by the National Key Basic Research Program of China (Grant No.2004CB117304);the National High Technology Development Program (Grant No.2006AA10A109-3,2004AA222090).

摘　　要：Using rapid amplification of cDNA ends （RACE）-PCR, two full-length cDNAs encoding putative seven-transmembrane receptors （designated Gh7TMpR1 and Gh7TMpR2） were cloned from cotton plants. Southern blot and an ApaL1 restriction site polymorphism analyses revealed that Gh7TMpR1 was derived from the ancestral A diploid genome, while Gh7TMpR2 was from the D subgenome. Northern blot hybridization indicated that both Gh7TMpR1 and Gh7TMpR2 were expressed preferentially in the elongation phase of fiber development. Majority of the Gh7TMpR1 proteins were located within the membrane structure and displayed a punctuate pattern of distribution. Overexpression of Gh7TMpR1 in fission yeast disrupted the polar growth and caused the formation of rounded cells. These results suggest that GhT7MpR1 may play a critical role in cotton fiber development, perhaps as a signaling receptor that is involved in controlling fiber elongation.用 cDNA 结束(种族) 的快速的扩大 -PCR, 编码通常认为的 seven-transmembrane 受体(指明的 Gh7TMpR1 和 Gh7TMpR2 ) 的二全身的 cDNAs 从棉花植物被克隆。南部的污点和地点多型性分析的 ApaL1 限制表明 Gh7TMpR1 从祖先的 A 被导出双染色体，当 Gh7TMpR2 从 D 潜水艇染色体时。北污点杂交显示 Gh7TMpR1 和 Gh7TMpR2 在纤维开发的延伸阶段优先地被表示。Gh7TMpR1 蛋白质的多数位于膜结构以内并且显示了分发的一个 punctuate 模式。在分裂酵母的 Gh7TMpR1 的 Overexpression 破坏了极的生长并且引起了圆细胞的形成。这些结果建议 GhT7MpR1 可以在棉花纤维开发起一个关键作用，也许，作为发信号的受体，那涉及控制纤维延伸。

关 键 词：COTTON Seven-transmembrane protein Fiber elongation Alloalleles 

分 类 号：S562[农业科学—作物学]