轴突成束和延伸蛋白ζ-1基因在1型和2型星形胶质细胞中的表达  

EXPRESSION OF FASCICULATION AND ELONGATION PROTEIN ZETA-1 IN TYPE-1 AND TYPE-2 ASTROCYTES

作　　者：何江虹[1] 张晔[1] 夏春林[1] 张万钰[2] 朱婷[2] 万明辉[1] 

机构地区：[1]苏州大学医学院解剖学教研室暨细胞神经生物学研究室,江苏苏州215123 [2]南通大学江苏省神经再生重点实验室,江苏南通226001

出　　处：《解剖学报》2008年第5期636-639,共4页Acta Anatomica Sinica

基　　金：国家自然科学基金资助项目(30772242);江苏省高校自然科学研究计划(05KJB310121)资助项目

摘　　要：目的探讨轴突成束和延伸蛋白ζ-1(FEZ1)基因在1型和2型星形胶质细胞(T1A、T2A)中的表达差异,及其在新生SD大鼠中枢神经系统中的表达。方法培养、分离、纯化T1A和T2A,提取总RNA,分别以Cy3-dCTP和Cy5-dCTP标记T1A和T2A的mRNA,进行基因表达谱芯片检测分析。选取其中与轴突延伸相关的FEZ1基因,应用半定量RT-PCR法研究FEZ1在新生大鼠中枢神经系统多个部位的表达。结果T1A和T2A中差异表达基因138条,其中60条在T1A中高表达,78条在T2A中高表达。FEZ1基因在T2A中高表达。RT-PCR结果显示,FEZ1在新生大鼠中枢神经系统大脑皮层、嗅球和脊髓组织中均有表达。结论T1A和T2A中存在多个差异表达基因,其中与轴突延伸相关的FEZ1基因表达明显差异,提示T1A和T2A可能在诱导轴突生长方面有不同功能;FEZ1基因与中枢神经系统的发育和再生相关。Objective To observe and analyze the expression of fasciculation and elongation protein zeta-1 （FEZ1） gene in purified type-1 astrecytes （T1A） and type-2 astrocytes （T2A）. Methods By the technique of gene-chip, some related genes in the different gene expression profiles of purified T1A and T2A were compared and analyzed. Increased mRNA levels of FEZ1 in T2A was further confirmed by RT-PCR. Results 138 different genes were differentially expressed in T1A and T2A, 60 of which were highly expressed in T1A and 78 in T2A. The expression of FEZ1 was up-regulated in T2A, and the expression of FEZ1 was detected in the different parts （cerebral cortex, olfactory bulb and spinal cord ） of the central nervous system of P1 rats. Conclusion FEZ1 gene is among several genes that expressed differently in T1A and T2A. The expression of FEZI is found in central nervous system of P1 rats. FEZ1 may play an important role in the development and regeneration of central nervous system.

关 键 词：轴突成束和延伸蛋白ζ-1 星形胶质细胞 胶质细胞谱系 基因表达谱芯片 反转录-聚合酶链式反应 大鼠 

分 类 号：R322.81[医药卫生—人体解剖和组织胚胎学] Q786[医药卫生—基础医学]