槲皮素和乙酸龙脑酯对细菌脂多糖诱导流产孕小鼠子宫组织中CD4^+/CD8^+T细胞的影响  

INFLUENCE OF QUERCETIN AND BORNYL ACETATE ON CD4^+/CD8^+T LYMPHOCYTES IN THE UTERUS FOLLOWING LPS-INDUCED ABORTION IN PREGNANT MICE

作　　者：王晓丹[1] 史万玉[1] 马爱团[2] 姜国均[2] 张建楼[2] 翟向和[2] 钟秀会[1] 

机构地区：[1]河北农业大学动物科技学院,河北保定071001 [2]河北农业大学中兽医学院,河北定州073000

出　　处：《解剖学报》2008年第5期734-737,共4页Acta Anatomica Sinica

基　　金：国家自然科学基金(30571361)资助

摘　　要：目的探讨小鼠子宫组织中CD4+和CD8+T细胞在早期胚胎丢失中的意义,研究槲皮素和乙酸龙脑酯对细菌脂多糖(LPS)诱导流产小鼠的保胎效果和对母胎界面免疫平衡的调节作用。方法采用LPS尾静脉注射,制造小鼠流产模型,孕4~7d分别口服不同保胎药物,用药前后检测各组(n=10)小鼠子宫组织CD4+/CD8+T细胞亚群的变化。结果LPS促流产后,小鼠子宫壁CD4+T细胞数量显著增多(P<0.01),CD8+T细胞无明显变化(P>0.05),CD4+/CD8+比值显著升高(P<0.01)。预先口服保胎药物能不同程度抑制LPS的作用。其中以槲皮素和乙酸龙脑酯联合使用组的保胎效果最为明显,CD4+/CD8+比值下降,较LPS流产模型组差异极显著(P<0.01)。结论小鼠子宫组织CD4+/CD8+比值升高与早期胚胎丢失关系密切,槲皮素和乙酸龙脑酯能够调节小鼠子宫局部的免疫微环境,从而起到一定的促孕保胎作用。Objective To investigate the significance of CD4^＋ and CD8^＋ T lymphocytes in the uterus in early embryo loss （or resorption）, and to elucidate the anti-abortive effect and the immunological modulation of the maternal-fetal interaction with quercetin and bornyl acetate. Methods Lipopolysaccharide （LPS）（0.10ug/mouse）was injected via the tail vein in order to induce abortion in 7-day-gestation mice which also received quercetin and bornyl acetate at days 4-7 of gestation. Uterine CD4^＋ and CD8^＋ T lymphocytes of each group （ n = t0） were detected by immunohistochemistry. Results The amount of CD4^＋ T lymphocytes in the uterus of LPS-induced abortion mice was much higher （ P 〈 0.01 ） than that of the control mice, but there was no significant difference in the amount of CD4^＋ T lymphocytes （P 〉 0.05）. Also, the rate of CD4^＋/CD8^＋ was increased significantly （ P 〈 0.01 ）. When quercetin and bornyl acetate was used to prevent LPS-induced abortion, fewer CD4^＋ T cells were counted. The effect of quercetin combined with bornyl on anti-LPS-induced abortion was more significant, and the rate of CD4^＋/ CD8^＋ was decreased to 0. 562 ± 0. 021, lower than that of LPS-abortion group （ P 〈 0.01 ）. Conclusion The increase of CD4^＋ / CD8^＋ T cells in the LPS-treated mouse uterus could be associated with the embryo loss, and quercetin and bornyl acetate have an anti-abortive effect through modulation of maternal-fetal interface immune balance.

关 键 词：胚胎丢失 CD4^+/CD8^+T细胞 细菌脂多糖 免疫组织化学 小鼠 

分 类 号：S852.1[农业科学—基础兽医学]