兔卵巢组织冷冻移植后卵母细胞成熟及受精发育能力的研究  

CAPABILITY OF MATURATION,FERTILIZATION AND FURTHER DEVELOPMENT OF OOCYTES RETRIEVED FROM RABBIT OVARIAN TISSUE TREATED WITH CRYOPRESERVATION AND AUTOLOGOUS TRANSPLANTATION

作　　者：晁岚[1] 邓晓惠[1] 李晓梅[1] 于璇[1] 于红玲[1] 甄军晖[2] 傅庆诏[1] 

机构地区：[1]山东大学齐鲁医院不孕不育诊疗中心,济南250012 [2]山东大学齐鲁医院病理科,济南250012

出　　处：《解剖学报》2008年第5期742-746,共5页Acta Anatomica Sinica

基　　金：山东省中青年科学家科研奖励基金资助项目(2005BS03001)

摘　　要：目的探讨兔卵巢组织冷冻复苏后移植对卵母细胞生长、成熟、受精发育潜能的影响。方法25只性成熟新西兰雌兔随机分为对照组(5只)、新鲜移植组(10只)及冷冻复苏移植组(10只),卵巢组织均自体多点移植于子宫系膜内。卵巢移植90d后对兔联合使用促排卵、体外成熟培养和卵泡浆内单精子注射技术,观察卵子的成熟情况以及体内和体外成熟卵子的受精率、卵裂率和囊胚形成率。结果新鲜移植组和冷冻复苏移植组获卵数均低于对照组(P<0.05),而两移植组间获卵数差异无显著性;各组未成熟卵子所占比率以及未成熟卵子体外成熟后的成熟率间均无显著性差异;各组间体内成熟卵子、体外培养成熟卵子的受精率、卵裂率和囊胚形成率的差异不明显;各组内体内成熟卵子与体外培养成熟卵子相比受精率、卵裂率间无显著性差异,但体外培养成熟卵子囊胚形成率明显低于体内成熟卵子(P<0.05)。结论兔卵巢经冷冻复苏自体移植后,其卵泡能够重新生长发育;通过体外成熟和受精技术,体内和体外成熟卵子均可产生正常的胚胎。提示卵巢组织冻融移植结合辅助生殖技术可作为一种生育力保存的方法。Objective To investigate the effects of transplantation of frozen-thawed ovarian tissues on the maturation, fertilization and further developmental potency of oocytes retrieved from grafts. Methods Twenty five New Zealand white female rabbits were divided into three groups randomly, group 1 （n = 5）, control group; group 2 （n = 10）, fresh ovarian tissues were autologously transplanted into the mesometrium; and group 3 （n = 10）, frozen-thawed ovarian tissues were autologously transplanted into the mesometrium. Three months after the transplantation, rabbits were stimulated with follicle-stimulating hormone and oocytes were retrieved from antral follicles 13 hours after human chorionic gonadotropin injection. In vitro maturation （IVM） and intracytoplasmic sperm injection （ICSI） were performed to evaluate the fertility potency of the oocytes from frozen ovarian grafts. Results The number of retrieved oocytes in group 2 and 3 were lower than those of the control group （ P 〈 0.05） ; But no significant differences were observed between group 2 and group 3 （ P 〉 0.05） ; There were no significant differences both in the percentage of immature oocytes and the maturation rate after IVM, among the 3 groups （ P 〉 0.05） ; Also, among the 3 groups or in each group, the fertilization rate, cleavage rate and blastocyst formation rate showed no difference, no matter the oocytes matured in vivo or in vitro （ P 〉 0.05） ; The blastocyst formation rate derived from oocytes that matured in vitro was significantly lower than oocytes that matured in vivo （ P 〈 0.05）. Conclusion Rabbit ovarian follicles can survive and reinitiate development after cryopreservation and autologous transplantation, and oocytes recovered from ovarian grafts can develop to normal embryos by IVM and ICSI. Thus, cryopreservation and transplantation of ovarian tissue and subsequent Assisted Reproductive Technologies provide possible means of fertility preservation.

关 键 词：卵巢 卵母细胞 冷冻保存 异位移植 兔 

分 类 号：R617[医药卫生—外科学]