雌激素受体β在小鼠及猪卵巢中表达的比较研究  

COMPARISON OF ESTRADIOL RECEPTOR β EXPRESSION IN THE OVARY OF MICE AND PIGS

作　　者：赵颖[1] 杨丹[1] 张岩[1] 刘姗姗[1] 李丹丹[1] 李树峰[1] 严云勤[1] 

机构地区：[1]东北农业大学组织胚胎学教研室,哈尔滨150030

出　　处：《解剖学报》2008年第5期747-750,共4页Acta Anatomica Sinica

基　　金：黑龙江省自然科学基金(C0327)资助项目

摘　　要：目的研究雌激素受体β(ERβ)在小鼠和猪卵巢中的表达差异。方法通过免疫组织化学的方法,研究ERβ在小鼠及猪卵巢中不同类型细胞中的表达量和细胞内的定位情况。结果在小鼠卵巢中,ERβ在黄体和各级生长卵泡的卵母细胞质中均有大量表达,在各级卵泡的颗粒细胞和膜细胞中表达量很少,生殖上皮和间质细胞中没有检测到表达信号。猪卵巢中ERβ强表达于各级卵泡的膜细胞中,各种直径的腔前卵泡的颗粒细胞中也有表达,但有腔卵泡颗粒细胞有的表达,有的则未见表达,间质细胞和生殖上皮中无明显表达。结论ERβ在小鼠和猪卵巢中的表达和定位差异很大,表明ERβ在小鼠和猪卵巢中的作用也可能不同。Objective To study the diversities of expression of estradiol receptor 13（ER~）in the ovary of mice and pigs. Methods Immunohistochemistry was used in the present study in order to detect the expression and distribution of estradiol receptor β in tissue section taken from both the mouse and pig. Results In the ovary of mouse, there was a higher intensity of immunohistochemical staining for ERβ in the corpora lutea and the cytoplasm of oocytes in all sizes of growth follicles, and a weaker intensity of immunohistochemical staining for ERβ in the granulose cells and thecal cells in all sizes of follicle. In addition, there was also a weaker intensity of immunohistochemical staining for ERβ in the interstitial cells and germinal epithelium. In the ovary of pig, there were highly intensity of immunohistochemical staining of ERβ in the thecal cells of follicles. Staining was also detected in the granulese ceils in the follicles before the formation of an antrum, but some of the granulose cells in the antrum follicle expressed ERβ, at the same time the others did not expressed it. There was no obvious expression in the interstitial cells or germinal epithelium. Conclusion There are great differences in the expression and distribution of estradiol receptor ERβ in the ovary of mice and pigs. These results indicate that ERβ may play very different roles in the ovary of mice and pigs.

关 键 词：雌激素受体Β 卵巢 免疫组织化学 猪 小鼠 

分 类 号：S852.1[农业科学—基础兽医学]