一种应用振荡切片显示神经元的Cox染色法  

AN IMPROVED COX METHOD FOR NEURON STAINING BY USING VIBRATOME SECTION

作　　者：汪薇曦[1] 杨世明[1] 叶翠芳[1] 李宏莲[1] 李和[1] 

机构地区：[1]华中科技大学同济医学院解剖学系组织胚胎学教研室,武汉430030

出　　处：《解剖学报》2008年第5期779-781,共3页Acta Anatomica Sinica

摘　　要：目的探索一种快捷、简便的大脑锥体细胞和小脑普肯耶细胞的染色方法。方法取猫、家兔、大鼠和豚鼠的大脑和小脑组织,升汞-重铬酸钾-铬酸钾固定液固定、浸染后,部分组织按传统的Cox法制片,即固定后进行火棉胶包埋、滑式切片机切片;部分组织用改良法制片,即组织不经包埋而直接进行振荡切片。结果与传统Cox法相比,应用振荡切片的Cox法减少了操作程序,大大缩短了制片时间,神经元染色均匀,神经元突起更为完整,小突起细节显示更为满意,且背景浅淡,非特异性沉淀颗粒极少。结论应用振荡切片的Cox改良法快捷、简便,神经元染色效果明显优于传统Cox法。Objective To explore a quicker and simpler method for the staining of cerebral pyramidal cells and cereberullarPurkinje cells. Methods Blocks of the cerebrums and cerebellums of the cat, rabbit, rat and guinea pig were removed, fixed and immersing-stained with a mixture of mercuric chloride, potassium bichremate and potassium chromate. Some of fixed blocks were embedded in collodion and sectioned on a sliding microtome. The sections were prepared by traditional Cox method. The other fixed blocks were cut on a vibratome without embedding, and the sections were prepared by the improved Cox method. Results Compared with Cox method, the improved method by using vibratome section had less procedure and took less time. Moreover, the staining of neurons by the improved method was more even, showing more complete processes and more details of small processes, with a lighter background and no or fewer if any, precipitations of staining reagents in the sections. Conclusion The improved Cox method by using vibratome section is quick and simple with higher successful rate and is superior in terms of neuron staining compared with the traditional Cox method.

关 键 词：神经元 改良 振荡切片 Cox染色法 猫 兔 大鼠 豚鼠 

分 类 号：R329-33[医药卫生—人体解剖和组织胚胎学]