胆固醇结石病人与正常人胆囊胆汁的蛋白质表达谱比较分析  

Differential analysis of protein profiles of gallbladder bile from patients with cholesterol cholelithiasis and controls

作　　者：张殿彩[1] 项建斌[1] 蔡端[1] 许志宾[2] 王丽影[2] 查锡良[2] 

机构地区：[1]复旦大学附属华山医院普外科复旦大学上海医学院外科学系,上海200040 [2]复旦大学上海医学院生物化学与分子生物学系,上海200032

出　　处：《中华肝胆外科杂志》2008年第9期636-639,共4页Chinese Journal of Hepatobiliary Surgery

基　　金：国家自然科学基金资助项目（30772111、30070737）和复旦大学“世纪之星”培养计划项目

摘　　要：目的比较分析胆固醇结石病人与正常人胆囊胆汁蛋白表达谱的差异，为阐明胆石成因提供实验依据。方法提取胆固醇结石病人和正常人胆囊胆汁总蛋白，构建相应的双向电泳蛋白质表达谱．直用ImageMaster图像分析软件获得差异蛋白点信息。结果胆固醇结石病人与正常人胆囊胆汁总蛋白浓度分别为（6．4824±0．3403）mg／ml和（2．3156±0．3064）mg／ml（P〈0．01）。两组组内蛋白质平均匹配点数分别为465±71和407±85．平均匹配率为71．70％和68．38％，组间269个蛋白质点相互匹配，匹配率为61．70％。胆固醇结石组蛋白表达谱显著差异点34个，16个表达上调，18个表达下调。结论初步建立了胆固醇结石病人和正常人胆囊胆汁蛋白表达谱，发现两者存在差异蛋白质点，为筛选胆石形成关键调控子提供基础。Objective To elucidate the mechanism of formation of cholesterol cholelithiasis, this study is designed to establish two-dimensional polyacrylamide gel electrophoresis system for differential analysis of the proteome of gallbladder bile from cholesterol eholelithiasis patients and control ones. Methods Total proteins of gallbladder bile were extracted from the subjects in two groups and the protein profiles were established hy using the immobilized pH gradient two-dimensional electrophoresis respectively. These maps were analyzed by ImageMaster two dimensional electrophoresis analysis software. Results The concentrations of bile proteins from cholesterol cholelithiasis patients and control ones were （6. 4824±0. 3403）mg/ml and （2. 3156±0. 3064）mg/ml（P〈0.01）, respectively. The average matched protein spots were 465 ± 71 and 407 ± 85 in the two groups, respectively. The matched rates were 71.70% and 68.38% correspondingly. There were 269 matched spots in the two reference gels maps and the matched rate was 61.70%. A total of 34 differential protein spots were identified, 16 expressed highly in cholesterol cholelithiasis patients but lowly in the control ones, while 18 expressed just in the opposite way. Conclusion The differential protein profiles of gallbladder bile between cholesterol cholelithiasis patients and controls have been established and several differential protein spots are found. The data may be a basis for further screening the key regulators of formation of cholesterol cholelithiasis.

关 键 词：胆结石 双向凝胶电泳 蛋白质组 胆汁 

分 类 号：R686[医药卫生—骨科学]