银翘散对流感病毒感染小鼠模型肺组织病毒载量和IFN-γ mRNA表达的影响  

Effects of Yinqiaosan on Influenza Virus Load and IFN-γ mRNA Expression in Mice

作　　者：时宇静[1] 赵晔[1] 姜晶[2] 郭姗姗[1] 苏丹[1] 黄洋[1] 高英杰[1] 崔晓兰[1] 

机构地区：[1]中国中医科学院中药研究所,北京100700 [2]航天中心医院,北京100049

出　　处：《中国药学杂志》2008年第19期1475-1478,共4页Chinese Pharmaceutical Journal

基　　金：国家十一五科技支撑项目(2006BAI08B04-02)

摘　　要：目的研究银翘散对流感病毒载量和IFN-γ mRNA表达的影响。方法除正常对照组小鼠不进行处理外,其他小鼠经甲型流感病毒感染后分为模型对照组,银翘散大、中、小剂量组。使用Real-time RT-PCR方法分别在流感病毒感染后第1,2,3,5,7天检测小鼠肺组织病毒载量和IFN-γ mRNA表达。结果银翘散可以降低肺组织病毒载量,其中大剂量组在各时间点均可降低病毒载量(P<0.05,P<0.01),中、小剂量组在第3天和第5天均可降低病毒载量(P<0.01,P<0.05);银翘散可以提高肺组织IFN-γ mRNA表达,其中大剂量组在各时间点均可提高IFN-γ mRNA表达(P<0.01,P<0.05),中剂量组在第2,3,5天可以提高IFN-γ mRNA表达(P<0.01),小剂量组在第3,5天可以提高IFN-γ mRNA表达(P<0.01)。结论银翘散可能通过上调流感病毒感染小鼠肺组织内IFN-γmRNA相对表达量,来发挥抗流感病毒的作用。OBJECTIVE To investigate the effects of Yinqiaosan on influenza virus load and IFN-γ mRNA expression. METHODS The mice were divided into control group, influenza virus group, Yinqiaosan high dose group, Yinqiaosan middle dose group and Yinqiaosan low dose group. Real-time PCR technique was used to detect virus load and IFN-γ mRNA expression in mice lung at the 1st day, 2nd day, 3rd day, 5th day and 7th day after bcing infected. RESULTS The high dose of Yinqiaosan decreased virus load at every time point（P 〈0. 05, P 〈0. 01 ）. The middle and low dose decreased virus load at the 3rd day and 5th day（P 〈 0. 01, P 〈0. 05）. Lung IFN-γ mRNA expression was increased in high dose group at every time point（ P 〈 0. 01, P 〈0. 05 ）, IFN-γ mRNA expression in middle dose group was increased at the 2nd day, 3rd day and 5th day（ P 〈 0. 01 ） , Yinqiaosan in low dose group can increase IFN-γ mRNA expression was increased in low dose group at 3rd and 5th day（P 〈0. 01 ）. CONCLUSION Yinqiaosan exerts antiviral effects against influenza virus by upregulaling expression of IFN-γ mRNA.

关 键 词：银翘散 甲型流感病毒 实时聚合酶链反应 病毒载量 Γ-干扰素 

分 类 号：R965[医药卫生—药理学]