HPLC同时测定心脑联通胶囊中葛根素、虎杖苷和野黄芩苷的含量  

Simultanous Determination of Puerarin,Polygonin and Scutellarin in Xinnaoliantong Capsules by HPLC

作　　者：栾爽[1] 王冬梅[1] 赵怀清[1] 高靥[1] 申涛[1] 石健[1] 

机构地区：[1]沈阳药科大学药学院,沈阳110016

出　　处：《中国药学杂志》2008年第19期1510-1512,共3页Chinese Pharmaceutical Journal

摘　　要：目的利用梯度洗脱,建立高效液相色谱法测定心脑联通胶囊中葛根素、虎杖苷和野黄芩苷含量的方法。方法采用高效液相色谱法。Diamonsil ODS柱(4.6mm×200mm,5μm),流动相为乙腈(A)和0.05%磷酸水溶液(B)梯度洗脱,流速1.0m L.min-1,检测波长336nm。结果葛根素的线性范围为0.0232～0.1856g.L-1(r=0.9995),平均回收率为100.0%,RSD=1.3%,虎杖苷的线性范围为0.0152～0.1216g.L-1(r=0.9996),平均回收率为98.7%,RSD=0.9%,野黄芩苷的线性范围为0.0046～0.0369g.L-1(r=0.9998),平均回收率为100.4%,RSD=0.9%。结论本方法操作简便,测定结果准确可靠,可用于心脑联通胶囊的质量控制。OBJECTIVE To develop a quantitative method simultanous for determination of puerarin, polygonin and scutellarin in Xinnaoliantong capsules by HPLC, METHODS The seperation was performed on a column Diamonsil ODS （4. 6 mm× 200 mm, 5 um） was used. The mobile phase was composed of acetonitrile（A） and 0. 05% H3PO4 （B） with gradient eluation. The flow rate was 1.0 mL · min^-1 with the wavelength at 336 nm. RESULTS For puerarin, the calibration curve was linear （ r = 0. 999 5 ） in the range of 0. 023 2 -0. 185 6 g· L^-1, the average recovery was 100. 0% （RSD = 1.3% ）. For polygonin, the calibration curve was linear （r=0.9996） in the range of 0.015 2-0.121 6g· L^-1, the average recovery was 98.7% （RSD=0.9%）. For seutellarin, the calibration curve was linear （r =0. 999 8） in the range of 0. 004 6 -0. 036 9g· L^-1, the average recovery was 100. 4% （RSD = 0. 9% ）. CONCLUSION The method was sensitive, accurate and can be used in the quality control of Xinnaoliantong capsules.

关 键 词：心脑联通胶囊 高效液相色谱法 葛根素 虎杖苷 野黄芩苷 

分 类 号：R917[医药卫生—药物分析学]