RP-HPLC法测定通光藤中11α-O-顺芷酰基-12β-O-乙酰基-通光藤苷元B的含量  

RP-HPLC determination of 11α-O-tigloyl-12β-O-acetyl-tenacigenin B in Marsdenia tenacissima(Roxb.)Wight et Arn

作　　者：王艳艳[1] 王威[1] 李红岩[1] 夏雪[1] 董方言[1] 

机构地区：[1]吉林省中医药科学院新药研究中心,长春130021

出　　处：《药物分析杂志》2008年第9期1409-1412,共4页Chinese Journal of Pharmaceutical Analysis

基　　金：国家自然科学基金资助项目(No.30772723)

摘　　要：目的:建立 RP-HPLC 法测定通光藤药材中11α-O-顺芷酰基-12β-O-乙酰基-通光藤苷元 B 的含量。方法:采用酸水解法降解通光藤 C_(21)甾体苷,以反相高效液相色谱法测定11α-O-顺芷酰基-12β-O-乙酰基-通光藤苷元 B 含量,色谱柱为 Diamonsil ODS(250 mm×4.6 mm,5 μm);柱温为16℃;流动相为乙腈-水(45:55);流速为0.8 mL·min^(-1);检测波长为220 nm。结果:11α-O-顺芷酰基-12β-O-乙酰基-通光藤苷元 B 进样量在1.016～5.080μg范围内与峰面积积分值呈良好的线性关系,r=0.9999(n=5);平均回收率(n=6)为98.8%,RSD=1.9%。结论:方法简便、快速、准确,为通光藤药材的质量评价和新药开发提供科学依据。Objective ： To establish an RP - HPLC method for qualitative analysis of 11α- O - tigloyl - 12β - O - acetyl - tenacigenin B in Marsdenia tenacissima. Method： The C21 steroidal glycosides were degraded into aglycones by a mild acid hydrolysis and 11α - O - tigloyl - 12β- O - acetyl - tenacigenin B was determined using an HPLC method. The Diamonsil ODS column （250 mm ×4. 6 ram,5 μm）was used with the mobile phase of acetonitrile - water（45：55 ） at a flow rate of 0. 8 mL · min^-1. The detection wavelength was 220 nm and the column temperature was 16 ℃. Results：The linear range of 11α - O - tigloyl - 12β - O - acetyl - tenacigenin B was 1. 016 -5.080 μg with the correlation coefficient 0. 9999 between the injection quantity and the peak area. The average recovery was 98. 82% with the RSD of 1.9% （n = 6）. Conclusion：The method is simple, convenient and accurate, which may be provided theoretical basis for quality assessment and new drug development of M. tenacissima.

关 键 词：通光藤 11α-O-顺芷酰基-12β-O-乙酰基-通光藤苷元B 反相高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]