HPLC法测定清眩降压片中芥子碱的含量  

HPLC determination of sinapine in Qingxuan Jiangya tablets

作　　者：翟宏宇 司云杉[2] 李银环[3] 

机构地区：[1]吉林省食品药品检验所,长春130033 [2]吉林省中医中药研究院,长春130021 [3]长春医学高等专科学校,长春130031

出　　处：《药物分析杂志》2008年第9期1551-1553,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立测定清眩降压片中芥子碱含量的方法。方法:采用高效液相色谱法,色谱柱为 Diamonsil C_(18)色谱柱(4.6 mm×250 mm,5μm),以乙腈-[0.1%磷酸溶液(每100 mL 加十二烷基磺酸钠0.1 g)](32:68)为流动相,流速为0.8 mL·min^(-1),检测波长为326 nm。结果:芥子碱进样量在0.02～0.25μg范围内与峰面积呈良好的线性关系(r=0.9999),平均回收率(n=6)为99.3%。结论:本方法操作简便,精密度好,结果准确可靠。Objective： To develop an HPLC method for determination of sinapine in Qingxuan Jiangya tablets. Methods：Analyses were carried out on Diamonsil C18 column（4.6 mm ×250 mm,5 μm） with a mobile phase of acetonitrile - [0.1% phosphoric acid（0.1 g sodium dodeeyl sulfate per 100 mL） 1 （32： 68）. The flow rate was 0.8 mL · min^- 1. UV detector wavelength was set at 326 nm. Results：The standard curve for sinapine was linear in the range of 0.02 - 0.25 μg （ r = 0. 9999 ）. The average recovery （ n = 6） was 99.3 %. Conclusion ： The method is convenient, rapid, accurate and reliable.

关 键 词：清眩降压片 芥子碱 含量测定 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]