罗丹明123经大鼠空肠黏膜的体外Ussing chamber渗透研究  

Ussing chamber technique for in vitro study of permeability of rhodamine 123 through rat jejunum mucosa

作　　者：黄蓓蓓[1] 任非[1] 李国锋[1] 王春霞[1] 李亦蕾[1] 孙亚彬[1] 

机构地区：[1]南方医科大学南方医院,广东广州510515

出　　处：《中国医院药学杂志》2008年第17期1440-1442,共3页Chinese Journal of Hospital Pharmacy

基　　金：2005年国家自然科学基金(编号:C03050205);2005年广东省科技计划项目(编号:2005B30101007);2006年笹川医学奖学金同学会科研启动基金(编号:101号)

摘　　要：目的:检测并评价罗丹明123(R123)经空肠黏膜渗透的特点,分析此法的可行性。方法:配制R123溶液,采用体外Ussing chamber实验法,考察R123经时经大鼠空肠黏膜吸收方向和分泌方向的累计透过量,并计算各自渗透系数。结果:R123吸收和分泌方向的表观渗透系数Papp(×10-5,cm.s-1)分别为(1.9±1.3)和(7.4±5.2),差异具显著性。结论:R123作为P-糖蛋白的底物,其经肠黏膜渗透以分泌方向为主;Ussing chamber实验用于评价药物在肠道内转运具有易操作,简便等特点,值得推广使用。OBJECTIVE To investigate the features of rhodamine 123 （R123） permeability through jejunum mucosa, and ana lyze feasibility of Ussing chamber in vitro. METHODS R123 solution was prepared. With in vitro Ussing chamber experiment , the accumulated amount of absorptive and secretory transport of R123 permeated through rat jejunum mucosa for was determined at different time points during the experiment, respectively. RESULTS The apparent permeability coefficient（Papp, × 10^-5 , cm·s^-1 ） of absorptive transport of rhodamine123 was （1.9 ± 1.3） significantly lower than that of secretory transport （7. 4 5.2）. CONCLUSION As the substrat of P-glycoprotein（P-gp）, the movement of R123 across the intestinal membranes was preferentially secretory direction; it is worth using Ussing chamber experiment because of the easy and feasible operation.

关 键 词：罗丹明123 体外Ussing CHAMBER 回肠 表观渗透系数 

分 类 号：R96[医药卫生—药理学]