巴戟天正丁醇提取物对乳鼠心肌细胞缺氧/复氧损伤后炎性细胞因子的影响  

Effect of the n-butanol extract of Morinda officinalis on inflammatory in neonatal rat cardiomyocytes by hypoxia/reoxygenation injury

作　　者：韩联合[1,2] 冯国清[2] 张贺鸣[2] 

机构地区：[1]郑州航空学院医院,河南郑州450015 [2]郑州大学医学院药理教研室,河南郑州450052

出　　处：《中国医院药学杂志》2008年第17期1450-1453,共4页Chinese Journal of Hospital Pharmacy

基　　金：河南省科技攻关项目(编号:0524410030)

摘　　要：目的:观察巴戟天正丁醇提取物对乳鼠心肌细胞缺氧/复氧损伤后炎性细胞因子的影响。方法:采用培养的乳鼠心肌细胞建立缺氧/复氧损伤模型,实验分正常细胞培养组、缺氧/复氧损伤模型组、巴戟天正丁醇提物小、中、大剂量组。平衡法测定TNF-α、IL-1β、IL-6的含量变化。结果:巴戟天正丁醇提物可明显改善缺氧、复氧损伤;降低TNF-α、IL-1β、IL-6的含量。结论:巴戟天正丁醇提取物对缺氧/复氧损伤的心肌细胞有防护作用;其防护作用可能与降低TNF-α、IL-1β、IL-6的含量有关。OBJECTIVE To evaluate the effects of the n-butanol extract of Morinda officinalis （BEM） on inflammatory cytokine TNF-α,IL-1β and IL-6 in cultured neonatal rat cardiomyocytes by hypoxia/reoxygenation injury. METHODS The models of hypoxia/reoxygenation were made with th first generation of cultured cardiomyocytes. Cultured cardiomyocytes of neonatal rats were divided into five groups：control group; hypoxia/reoxygenation （H/R） group; the groups of H/R ＋ low dose of BEM（0. 038 g·L^-1）, H/R＋ medium dose of BEM（0.11 g·L^-1） and H/R＋ high dose of （0. 34 g·L^-1 ）. The contents of cardiomyocytes TNF-α,IL-1β and IL-6 were tested by balanced technique. RESULTS Group BEM decreased more significantly the TNF-α,IL-1β and IL-6 levels than group model and group control（P〈0. 05）. CONCLUSION BEM has obviously protective effect in cultured neonatal rat cardiomyocytes by hypoxia/reoxygenation injury. The protective effect is , to some degree, related to the inhibition of TNF-α,IL-1β and IL-6.

关 键 词：巴戟天正丁醇提取物 心肌细胞培养 缺氧/复氧损伤 肿瘤坏死因子-α 白介素-1Β 白介素-6 

分 类 号：R96[医药卫生—药理学]