营养不良大鼠血浆小分子物质代谢组学的研究  

Metabonomics for plasma low-molecular metabolites of malnutrition in rats

作　　者：李民[1] 吴泽明[2] 李幼生[1] 许国旺[2] 李宁[1] 黎介寿[1] 

机构地区：[1]南京大学医学院临床学院 南京军区南京总医院解放军普通外科研究所,江苏南京210002 [2]中国科学院大连化学物理研究所,辽宁大连116023

出　　处：《肠外与肠内营养》2008年第5期259-263,共5页Parenteral & Enteral Nutrition

基　　金：国家重点基础研究发展规划资助(2003CB515502);南京军区医学科学技术研究“十一五”计划(06Z41)

摘　　要：目的:利用代谢组学方法检测能反应营养不良大鼠血浆小分子代谢物质,探讨代谢组学在营养不良研究中的应用。方法:实验分为营养不良组和对照组。对照组大鼠自由进食、饮水,营养不良组自由进水,每天给予该体质量大鼠所需食物的60%。造模成功后,收集大鼠血浆,用液质分析(LC-MS)方法检测并比较两组大鼠血浆小分子物质。结果:营养不良组大鼠与对照组相比,血浆中肉毒碱和色氨酸碎片含量增加,而甜菜碱、棕榈酰肉碱、亚麻酸、二十二碳六烯酸和花生四烯酸等含量均降低。结论:用LC-MS方法检测得到的小分子物质与大鼠营养不良相关。代谢组学可作为营养不良指标的筛选方法。Objective： The plasma low-molecular metabolites of malnutrition rats was detected through metabonomics to explore the fesibility of metabonomics in the application of malnutrition. Methods： Rats were randowly divided into control group and malnutrition group. The rats could drink water freedomly. 60% of standard food was given to malnutrition group rats and there was no restriction of food control group. After model of malnutrition was established, the plasma was collected and low-molecular metabolites was detected. Results： Compared to control group,the intensity of carnitine and tryptophan fragment were higher and the intensity of betaine, palmitoylcarnitine, linolenic acid, docosahexaenoic acid and arachidonic acid were lower. Conclusion： Low-molecular metabolites in plasma detected through metabonomics are related to nutrition status. Metabonomics can be recognized as an technique for evaluating malnutrition.

关 键 词：代谢组学 营养不良 大鼠 小分子物质 液质联用 

分 类 号：R591[医药卫生—内科学]