β-内啡肽与μ-阿片受体在正常皮肤与增生性瘢痕组织中的分布特征与生物学意义  

The significance and characteristics of distribution of β-endorphin and μ-opioid receptors in normal skin and hyperplastic scar

作　　者：程飚[1] 刘宏伟 李勤[1] 付小兵[3] 盛志勇[3] 曹荣[4] 

机构地区：[1]解放军广州军区广州总医院整形外科,广州510010 [2]暨南大学华侨医院整形外科,广州 [3]解放军总医院三0四临床部全军创伤修复重点实验室,北京 [4]解放军第四军医大学部全军神经科学研究所,西安

出　　处：《中华整形外科杂志》2008年第5期343-346,共4页Chinese Journal of Plastic Surgery

基　　金：国家重点基础研究发展计划资助项目（2005CB522603）;国家自然科学基金资助项目（30570575,30770702）;全军医学科学技术研究“十一五”计划面上项目（06MB160）

摘　　要：目的观察β-内啡肽和μ-阿片受体（MOR）在人正常皮肤与增生性瘢痕组织中的表达特点。方法采取10例正常人皮肤组织和10例痛痒症状明显的增殖期增生性瘢痕患者病变处皮肤与非病变处正常组织，运用免疫组织化学技术对所取标本进行β-内啡肽和MOR荧光染色，并应用RT-PCR方法检测皮肤组织中MORmRNA的表达情况。结果在所有被检测的皮肤组织标本中，真表皮内均发现β-内啡肽和MOR的阳性表达。其中正常人皮肤与瘢痕患者非病变处来源组织真皮与表皮内的β-内啡肽和MOR的表达差异无统计学意义（P〉0．05）；而增生性瘢痕患处组织真皮与表皮内β-内啡肽和MOR的表达明显强于其他两组（P〈0．01）。同时，PCR的结果显示，瘢痕组织中MORmRNA的含量也远远高于正常皮肤。结论β-内啡肽和MOR在正常皮肤与增生性瘢痕组织中的分布存在差异，这可能是造成瘢痕愈合后感觉异常的重要因素。Objective To detect the distribution of β-endorphin and mu-opioid receptors （MOR） in normal skin tissue and scar tissue from healthy volunteers and patients with hypertrophic scar. Methods Normal skin samples from 10 healthy individuals and 10 patients with hypertrophic scar, and scar samples from the same 10 patients were investigated. The β-endorphin and MOR protein in the samples were detected by immunofluorescence （IF） . The reverse transcription polymerase chain reaction （RT-PCR） was used to detect MOR mRNA. Results β-endorphin and MOR protein were expressed in all samples. There were no significant differences in the expression ofβ-endorphin and MOR protein between normal skin from healthy volunteers and patients with hypertrophic scar （ P 〉 0.05 ）. The expression of β-endorphin, MOR protein and mRNA in hyperplastic scar was significantly stronger than that in normal skin（ P 〈 0.01 ）. Conclusions The expression of β-endorphin and MOR is different in normal skin and hypertrophic scar. This maybe the possible reason of scar cacesthesia.

关 键 词：瘢痕 Β-内啡肽 阿片受体 感觉异常 

分 类 号：R622[医药卫生—整形外科]