细胞直接三维受控组装技术体外构建组织工程化软骨  

Direct construction of tissue-engineered cartilage by using three-dimension cell-assembly technique

作　　者：李曼[1] 赵铱民[1] 王小红[2] 颜永年[2] 秦海燕[1] 马佳[1] 

机构地区：[1]西安第四军医大学口腔医院,710032 [2]北京清华大学机械工程系激光快速成形中心,生命科学与医学研究院生物制造研究所

出　　处：《实用口腔医学杂志》2008年第5期621-623,共3页Journal of Practical Stomatology

摘　　要：目的:结合组织工程和生物制造(bio-manufacturing,BM)工程原理,研究应用快速成形(rapid prototy-ping,RP)技术中的细胞-材料直接三维受控组装技术体外构建组织工程化软骨的可行性。方法:提取兔耳软骨细胞与海藻酸钠和明胶均匀混合,调整细胞密度为3.0×107/ml。细胞基质材料混合物用细胞三维受控组装机Ⅰ代(清华大学机械系激光快速成形中心研制)按照预先设定的参数构建10 mm×10 mm×5 mm大小的软骨组织前体,分别在体外培养1、2、3、4周后对标本进行大体观察以及做冷冻切片进行组织学检测。结果:软骨细胞在结构体内增殖显著,保持正常的细胞形态和活力,组织学检测表明有大量软骨基质形成。结论:细胞直接三维受控组装技术为构建组织工程化软骨提供了一种可行的新途径。Objective：To fabricate a tissue-engineered cartilage by using three-dimension（3D） cell-assembly technique. Methods： Alginate and gelatin were mixed to form hydrogel, which was used as scaffold. Chondrocytes suspension was added into the hydrogel. Mixture of hydrogel and chondrocytes was used to fabricate tissue-engineered cartilage by 3 D cell-assembly technique. After being cultured in vitro for 1 -4 weeks, the samples were observed by histological methods. Results：The chondrocytes in the 3D cartilage could grow, proliferate and maintain the chondrocytes phenotype and activity. Conclusion ：The 3D cell-assembly technique is a very good technique to fabricate tis- sue-engineered cartilage.

关 键 词：组织工程 细胞组装 软骨细胞 海藻酸钠 

分 类 号：R318.1[医药卫生—生物医学工程]