光子嫩肤术对大鼠皮肤胶原的影响  

Effects of intense pulsed light on collagens in rat skin

作　　者：朱慧琴[1] 周萍英[1] 陈玲娣[1] 黄一雄[2] 沈尊理[2] 谢匡成[3] 

机构地区：[1]上海交通大学附属第一人民医院皮肤科,上海200080 [2]上海交通大学附属第一人民医院整形外科,上海200080 [3]上海交通大学附属第一人民医院中心实验室,上海200080

出　　处：《中国美容医学》2008年第9期1330-1333,共4页Chinese Journal of Aesthetic Medicine

摘　　要：目的:研究光子嫩肤术(IPL)对大鼠皮肤Ⅰ型胶原、Ⅲ型胶原及其基因表达的影响,探讨IPL的作用机理。方法:选择24只SD大鼠,每只背部皮肤选左右2个部位,右侧对照,左侧用强脉冲光照射,按实验时间段分成1周、2周、4周、8周四组,在照射后分别切取实验及对照部位皮肤作组织学观察,包括HE染色、Masson胶原染色;用实时荧光定量逆转录-聚合酶链反应(RT-PCR)法检测Ⅰ型、Ⅲ型胶原mRNA表达。结果:照射后1周、2周,两组皮肤的胶原含量无明显区别;照射4周、8周实验侧皮肤胶原纤维明显增多,同时,实验侧mRNA的表达在4周、8周均比对照侧增强(P<0.05)。结论:IPL能促进大鼠皮肤新生胶原的增生。Objective To study the effects of intense pulsed light（IPL） on collagens and mRNA expression of collagenⅠ , collagen Ⅲ in rat skin, and elucidate the mechanism of IPL. Methods 24 SD rats were irradiated on their left sides of the back with IPL, and their right sides were selected as control areas. We divided rats into four groups by time of irradiation. Skin specimens were taken both from treated and control areas after 1 week, 2 weeks, 4weeks and 8 weeks IPL treatments. Histological changes were observed by hematoxylin-eosin-staining, Masson trichrome staining, and mRNA expression levels of collagen Ⅰ and collagen Ⅲ were detected by real-time quantitative reverse transcription polymerase chain reaction（RT-PCR）. Results No obvious changes were seen at 1 week, 2 weeks. At week 4 and week 8, the staining of collagen was stronger in treated areas than in control areas, and the mRNA expression levels of collagens were also increased at week 4 and week 8 （P〈0.05）. Conclusion IPL can increase collagen production in rat skin.

关 键 词：光子嫩肤术 Ⅰ型胶原 Ⅲ型胶原 大鼠 实时荧光定量逆转录-聚合酶链反应 

分 类 号：Q682[生物学—生物物理学]