Construction of a chloroplast protein interaction network and functional mining of photosynthetic proteins in Arabidopsis thaliana  

作　　者：Qing-Bo Yu Guang Li Guan Wang Jing-Chun Sun Peng-Cheng Wang Chen Wang Hua-Ling Mi Wei-Min Ma Jian Cui Yong-Lan Cui Kang Chong Yi-Xue Li Yu-Hua Li Zhongming Zhao Tie-LiuShi Zhong-Nan Yang 

机构地区：[1]College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China [2]Shanghai Information Center for Life Science, Shanghai Institutes for Biological Sciences, The Chinese Academy of Sciences, Shanghai 200032, China [3]National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, The Chinese Academy of Sciences,Shanghai200032, China [4]College of Life Sciences, The Northeast Forestry University, Harbin, Heilongjiang 150040, China [5]Research Center for Molecular and Developmental Biology, Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, The Chinese Academy of Sciences, Beijing 100093, China [6]Department of Psychiatry and Center for the Study of Biological Complexity Virginia Commonwealth University, Richmond, VA 23298, USA [7]Bioinformation Center, Key Laboratory, of System Biology, Shanghai Institutes for Biological Sciences, The Chinese Academy of Sciences, Shanghai 200232, China

出　　处：《Cell Research》2008年第10期1007-1019,共13页细胞研究（英文版）

基　　金：Acknowledgements We thank the RIKEN BRC in Japan for provision of all full-length cDNA in this study. National Natural Science Foundation of China （grants numbers 30530100 and 90408010）, the State Key Program of Basic Research of China （grant numbers 2007CB947600 and 2007CB108800）, and Hi-Tech Research and Development Program of China （grant number 2006AA02Z313） supported this project.

摘　　要：Chloroplast is a typical plant cell organelle where photosynthesis takes place. In this study, a total of 1 808 chloroplast core proteins in Arabidopsis thaliana were reliably identified by combining the results of previously published studies and our own predictions. We then constructed a chloroplast protein interaction network primarily based on these core protein interactions. The network had 22 925 protein interaction pairs which involved 2 214 proteins. A total of 160 previously uncharacterized proteins were annotated in this network. The subunits of the photosynthetic complexes were modularized, and the functional relationships among photosystem Ⅰ （PSI）, photosystem Ⅱ （PSII）, light harvesting complex of photosystem Ⅰ （LHC Ⅰ） and light harvesting complex of photosystem Ⅰ （LHC Ⅱ） could be deduced from the predicted protein interactions in this network. We further confirmed an interaction between an unknown protein AT1G52220 and a photosynthetic subunit PSI-D2 by yeast two-hybrid analysis. Our chloroplast protein interaction network should be useful for functional mining of photosynthetic proteins and investigation of chloroplast-related functions at the systems biology level in Arabidopsis.

关 键 词：ARABIDOPSIS chloroplast protein network functional linkage PHOTOSYNTHESIS 

分 类 号：Q945.11[生物学—植物学]