原发性小肠腺癌中钙粘蛋白基因异常甲基化及蛋白表达的研究  

E-cadherin gene hypermethylation and its protein expression in primary adenocarcinomas of the small bowel

作　　者：李小燕[1] 黄缘[1] 宫亮[2] 

机构地区：[1]南昌大学第二附属医院消化科江西省重点分子医学实验室,330006 [2]南昌大学第四附属医院消化科

出　　处：《中华消化杂志》2008年第8期513-516,共4页Chinese Journal of Digestion

摘　　要：目的 探讨原发性小肠腺癌中钙粘蛋白（E—cad）基因甲基化状态和蛋白表达的特点，及其与小肠腺癌临床病理特征的关系。方法分别采用甲基化特异性PCR（MSP）和免疫组化SP法检测36例原发性小肠腺癌及相应癌旁组织中E-cad基因甲基化状态及蛋白表达水平，并结合临床资料进行分析。结果小肠腺癌和癌旁组织中E—cad甲基化率分别为38．9%（14／36）和8．3％（3／36），差异有统计学意义（P〈0．01）。小肠腺癌和癌旁组织中E—cad蛋白的阳性率分别为41．7％（15／36）和97．2％（35／36），差异有统计学意义（P〈0．01）。E—cad蛋白缺失的21例标本中有12例发生甲基化，与蛋白缺失相关（P〈0．01）。E-cad甲基化率及蛋白表达率与患者性别、年龄、肿瘤大小、发生部位无关（P〉0．05），与肿瘤浸润深度、分化程度及淋巴结转移密切相关（P〈0．05）。结论E—cad基因的异常甲基化可影响蛋白表达，与小肠腺癌浸润深度、分化程度及淋巴结转移有关。Objective To investigate the methylation status of E-cadherin （E-cad） and its protein expression in human small bowel adenocarcinomas （SBAC） , and the association of these findings with clinico-pathological characteristics. Methods E-cad gene hypermethylation and its protein expression in 36 patients with primary SBAC and the paired adjacent tissues were tested by methylation-specific PCR （MSP） assay and immunohistochemistry （IHC）respectively. Data associated with clinieo-pathological characteristics was analyzed. Results The hypermethylation frequency of E-cad was 38. 9% in cancerous tissues and 8.3 % in adjacent tissues （P 〈0.01）. The positive rate of E-cad expression was 41.7% in cancerous tissues and 97. 2% in adjacent tissues （P 〈0. 01）. Twelve out of 21 cases without protein expression revealed methylated（ P〈0.01 ）. Methylation status was closely related to E-cad protein expression in SBAC. The hypermethylation of E-cad gene and the expression of E-cad protein were significantly correlated with the depth of invasion, the differentiation and lymph node metastasis （P〈0.05 ） , but were not correlated with other clinico-pathologic parameters such as age, sex, tumor size or location（ P〉0.05）. Conclusions The hypermethylation of E-cad gene can downregulate the protein expression, which closely related to the depth of invasion, the differentiation and lymph node metastasis.

关 键 词：肠肿瘤 腺癌 钙粘蛋白 

分 类 号：R686[医药卫生—骨科学]