Tribbles在人单核源性巨噬细胞中的表达及氧化低密度脂蛋白对其的调节作用  

Expression of Tribbles in human monocyte-driven macrophages and the regulative role of ox-LDL on them

作　　者：尚嫄嫄[1] 张薇[1] 张立平[2] 邓景惕[2] 唐梦熊[1] 钟明[1] 张运[1] 

机构地区：[1]教育部和卫生部心血管重构和功能研究重点实验室山东大学齐鲁医院心内科 [2]山东大学医学院解剖学教研室,济南250012

出　　处：《山东大学学报（医学版）》2008年第9期833-836,共4页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金资助课题(30670874);山东省自然科学基金资助课题(Y2005C11)

摘　　要：目的探讨人单核源性巨噬细胞中Tribbles(TRBs)的表达及氧化低密度脂蛋白(ox-LDL)对TRBs(TRB1、TRB2、TRB3)表达的调节。方法体外培养人单核源性巨噬细胞,应用ox-LDL处理自然分化的人单核源性巨噬细胞,采用Real-Time PCR检测人单核源性巨噬细胞TRBs mRNA的表达情况及其不同浓度的ox-LDL、低密度脂蛋白(LDL)处理不同的时间后三种TRBs mRNA的表达。结果人单核源性巨噬细胞中TRBs的表达以TRB3为主,TRB1、TRB2微量表达;ox-LDL能促进TRB2和TRB3表达,减低TRB1表达。与LDL对照组相比,50μg/mL ox-LDL刺激巨噬细胞后,TRB1 mRNA的表达减少70%(P<0.01),TRB2 mRNA的表达增加5.2倍(P<0.01),TRB3 mRNA增加3.5倍(P<0.05),并且这种调节作用均呈剂量和时间依赖性。结论本研究证实了TRBs基因在人单核源性巨噬细胞中有表达,ox-LDL能调节巨噬细胞TRBs mRNA的表达,推测TRBs特别是TRB3可能参与了ox-LDL诱导的巨噬细胞凋亡过程。Objective To investigate the expression of Tribbles （TRBs） in human monocyte-driven macrophages and to analyze the regulative role of oxidized low density lipopmtein （ox-LDL） on TRB1, TRB2 and TRB3. Methods Primary human mono- cyte-driven macrophages were cultured in vitro. Macrophages were incubated with ox-LDL and low density lipoprotein （LDL） at various concentrations for various times. The level of TRBs mRNA was determined by quantification real-time RT-PCR. Results All three TRBs genes （TRB1, TRB2 and TRB3） were expressed in human monocyte-driven macrophages, among them, TRB3 was predominant, and TRB1 and TRB2 were little expressed. Ox-LDL increased the levels of TRB3 andTRB2 mRNA, while it decreased the expression of TRB1 in a dose- and time-dependent manner. Conclusions All three TRBs are expressed in human monocyte-driven macmphages and can be regulated by ox-LDL, so TRBs, especially TRB3 may participate in the course of mac- rophage apoptosis.

关 键 词：基因 Tribbles 巨噬细胞 氧化低密度脂蛋白 

分 类 号：R543[医药卫生—心血管疾病]