兔急性脊髓损伤后磁刺激运动诱发电位与病理变化的相关性  

Transcranial magnetic stimulation motor evoked potentials and pathologic changes in rabbit spinal cord injury

作　　者：侯勇[1] 聂林[1] 吴琦[2] 邵军[3] 吕丽红[3] 

机构地区：[1]山东大学齐鲁医院骨创科,济南250012 [2]山东大学医学院解剖学教研室,济南250012 [3]山东大学医学院手术学教研室,济南250012

出　　处：《山东大学学报（医学版）》2008年第9期855-858,共4页Journal of Shandong University:Health Sciences

摘　　要：目的观察兔急性脊髓损伤后经颅磁刺激运动诱发电位(transcranial magnetic stimulation motor evoked ponten-tials,TMS-MEP)的变化规律,并与脊髓病理变化相比较,探讨其相关性。方法将日本大耳兔32只随机分为4组,每组8只。A组为对照组,B、C、D组为实验组(脊髓损伤组)。然后对每组动物分别于麻醉后、暴露脊髓后、伤后5 min3、0 min1、h6、h、1 d、3 d、7 d检测TMS-MEP,对所得波形数据进行统计学分析。于伤后1 d3、d、7 d采用后肢的Tarlov分级行运动功能评分。以损伤部位为中心切取脊髓标本,行HE染色光镜、电镜观察。结果随着脊髓损伤的加重,MEP的潜伏期逐渐延长,波幅在逐渐减小,病理显示白质纤维脱髓鞘越重,神经细胞损伤数目越多,MEP的潜伏期延长和波幅降低越明显。结论经颅磁刺激运动诱发电位与脊髓病理变化有明显的相关性,TMS-MEP对脊髓损伤十分敏感,能客观地检测脊髓运动功能,反映脊髓损伤程度。Objective To explore the changes of transcranial magnetic stimulation motor evoked potentials（TMS-MEP） and spinal cord pathology in rabbit spinal cord injury, and to explore their correlation. Methods A digital weight drop apparatus induced spinal cord lesion was performed to mimic human SCI. Based on the drop height, 32 rabbits were randomly divided into four groups： the control, 2 cm, 4 cm and 6 cm injured groups. TMS-MEP was used to assess neurologic function. The MEP was recorded at 5 min, 15 min, 30 min, 60 min, 6 h, 24 h, 3 d, 7 d post-injury and before spinal cord injury in injury groups. Tarlov behaviour score was performed before and after surgery at 1, 3, 7 days. Light and electron microscope were used to observe the pathological changes. Results Spinal cord injmy resulted in a gradual decrement in the peak latency and significant increment in the peak amplitude. Simultaneously, histological studies showed that the drop causes a ischemia lesion, which was found in human spinal cord injury. Conclusion TMS-MEP has a significant association with spinal cord pathological changes and will be a noninvasive examination for spinal cord function.

关 键 词：经颅磁刺激 运动诱发电位 脊髓损伤 病理 兔 

分 类 号：R322.81[医药卫生—人体解剖和组织胚胎学]