基因工程菌对偶氮染料脱色及生物强化作用  

Decolorization and bioaugmentation on azo dye using gene engineering strain Escherichia coli JM109(pGEX-AZR)

作　　者：金若菲[1] 周集体[1] 王竞[1] 张爱丽[1] 

机构地区：[1]大连理工大学环境与生命学院,辽宁大连116024

出　　处：《大连理工大学学报》2008年第5期646-650,共5页Journal of Dalian University of Technology

基　　金：辽宁省自然科学基金资助项目(20032122)

摘　　要：研究了基因工程菌Escherichia(E.)coliJM109(pGEX-AZR)对偶氮染料的脱色及生物强化能力.实验表明,含有10%E.coliJM109(pGEX-AZR)的强化体系对酸冲击的耐受能力没有提高,脱色率仅为80%;而对碱度及盐度的冲击表现出较强的耐受能力,脱色率超过90%.同时在AnSBRs中连续运行42 d,强化体系耐浓度冲击的能力和脱色率均高于对照体系.利用RISA测定微生物群落结构,E.coliJM109(pGEX-AZR)在强化体系中始终作为优势菌群存在并保持较高的代谢活性.Decolorization and bioaugmentation on azo dye are investigated by using gene engineering strain Escherichia （E.）coli JM109 （pGEX-AZR）. It is shown that the tolerance to acid shock of the bioaugmentation system with 10% E. coli JM109 （pGEX-AZR） is not enhanced, and the colorific removal is only 80%. High tolerance is observed to alkali and salt shock. The colorific removal exceeds 90%. Continuous operating in AnSBRs for 42 d, both the tolerance to concentration shock and the colorifie removal are higher than that of the control system. Changes in microbial community structures can be detected by RISA, the introduced E. coli JM109 （pGEX-AZR） is persistent in the augmented systems and maintains high metabolic activity.

关 键 词：ESCHERICHIA COLI JM109(pGEX-AZR) 偶氮染料 生物脱色 生物强化 RISA 

分 类 号：X52[环境科学与工程—环境工程]