金匮肾气丸、右归丸对肾阳虚小鼠模型影响的脑基因图谱研究  

Gene expression of brain in mice with kidney-yang deficiency treated by Jinguishenqi Pills or Yougui Pills

作　　者：杨裕华[1] 李震[2] 陶汉华[2] 

机构地区：[1]山东省疾病预防控制中心,山东250014 [2]山东中医药大学

出　　处：《北京中医药大学学报》2008年第9期600-607,共8页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(No.30672573)

摘　　要：目的在基因水平探讨补肾中药金匮肾气丸、右归丸对"劳倦过度、房室不节"肾阳虚小鼠模型影响的药物作用机理及不同方剂引起基因改变的比较。方法对照组、造模组、金匮肾气丸组和右归丸组,各组雄性小鼠分别为10、15、10和10只,均予正常喂养。对照组和造模组每天灌胃蒸馏水0.5mL/只,治疗组每天分别灌胃金匮肾气丸混悬液0.5mL/只和右归丸混悬液0.5mL/只,造模组和治疗组均采用雄雌鼠比例1:6同笼喂养和雄鼠每日游泳30～40min达4周,以诱导产生"劳倦过度、房室不节"肾阳虚证。用36K mouse genome array鼠脑基因芯片检测各组鼠脑基因。结果造模组共筛选出186个差异表达基因,其中上调基因150个,下调基因36个。金匮肾气丸组筛选出299个差异表达基因,其中上调基因114个,下调基因185个。右归丸组筛选出155个差异表达基因,其中上调基因64个,下调基因91个。两治疗组均上调的基因24个,分别占各组上调基因的21.05%和37.50%,主要是与神经传递/信号转导、转录/翻译、激素、细胞周期和代谢相关的基因;均下调的有35个基因,分别占各组下调基因的18.92%和38.46%,主要是与转录/翻译、神经传递/信号转导、炎症/免疫、代谢以及影响多巴胺生成限速酶有关的基因。造模组上调基因而治疗组下调基因的有23个基因,主要是与炎症/免疫、神经传递/信号转导等相关基因;造模组下调基因而治疗组上调基因的基因有6个,主要是与细胞周期/细胞结构、神经传递/信号转导、转录等有关基因。结论金匮肾气丸、右归丸可使肾阳虚小鼠模型显著下调的激素、黑色素显著上调,并促进细胞增殖,从而在基因水平上探讨了金匮肾气丸和右归丸的药物作用机理及其差别。Objective To inquire into the cerebral gene changes in mice with kidney-yang deficiency caused by overwork and intemperance in sexual activity following the treatment of Jinguishenqi Pills（JP） or Yougui Pills（YP）. Methods Forty-five male mice were divided into 4 groups, with 10 in the control group, 15 in the model group, 10 in the JP group and 10 in the YP group. Every mouse in the control and model group was given distill water 0.5 mL per day, and every mouse in the JP and YP group were given 0.5 mL suspension of JP or YP per day. The male mice in the model, JP and YP group lived in the same cage with female mice at the ratio of 1 ： 6 （ male ： female）, and swam 30 - 40 min everyday for 4 weeks to result in kidney-yang deficiency caused by overwork and intemperance in sexual activity. The brain gene expression of the mice were detected with mouse brain gene chip of 36K mouse genome array made by CapitalBio Corp. Beijing, China. Results Compared with the control group, 186 differential expression genes were screened in the model group, including 150 up-regulated genes and 36 downgenes; 299 differential expression genes were screened in the JP group, including 114 up-regulated genes and 185 down-regulated genes; 155 differential expression genes were screened in the JP group, including 64 up-regulated genes and 91 down-regulated genes. There were 24 up-regulated genes both in the JP and YP group, accounting for 21.05 percent and 37.5 percent in each group respectively, which were mainly the genes related to neurotransmissions/signal transduction, transcription/translation, cellular cycle, hormone and metabolism. The down-regulated genes both in the GP and YP group were 35, accounting for 18.92 percent and 38.46 percent in each group respectively, chiefly including the genes associated with inflammation/immunization, neurotransmissions/signal transduction, transcription/ translation, metabolism and limiting velocity enzyme of dopamine. Compared with the control group and model group respectively, th

关 键 词：DNA芯片 肾阳虚 动物模型 金匮肾气丸 右归丸 小鼠 

分 类 号：R241.6[医药卫生—中医诊断学]