经前期综合征肝气逆证模型大鼠海马γ-氨基丁酸A受体β_2亚基mRNA表达  

Expression of GABAA receptor subunit β_2 mRNA in hippocampus of pre-menstrual syndrome model mice with syndrome of reversed liver qi

作　　者：张惠云[1] 王杰琼[1] 刘恒[1] 

机构地区：[1]山东中医药大学,山东250355

出　　处：《北京中医药大学学报》2008年第9期608-610,共3页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(No.30572291)

摘　　要：目的探讨大鼠海马γ-氨基丁酸A受体(GABAA受体)β2亚基mRNA表达的变化与经前期综合征肝气逆证的关系。方法采用半定量RT-PCR基因扩增技术对正常组、经前期综合征肝气逆证模型组和白香丹胶囊给药组大鼠海马中GABAA受体β2亚基的表达变化进行检测。结果与正常组大鼠相比,模型组大鼠海马GABAA受体β2亚基mRNA表达显著升高(P<0.05),调肝方药白香丹胶囊干预能改变该受体表达水平异常升高的状态。结论经前期综合征肝气逆证的发病机制与海马GABAA受体β2亚基有关。Objective To study the relationships of pre-menstrual syndrome （PMS） and expression of GABAA receptor β2 subunit mRNA in hippocampus. Method The expression of CABAA receptor β2 subunit mRNA in rat hippocampus was detected by semi-quantitative RT-PCR between normal group, model group and Baixiangdan Capsules group. Result Compared with the normal group, the expression of GABAA receptor β2 subunit mRNA was significantly high in hippocampus of the model group, and Baixiang dan Capsules decreased the high expression of it. Conclusion The pathogenesis of PMS with the syndrome of reversed liver qi is concerned with GABAA receptor subunit β2 in hippocampus.

关 键 词：经前期综合征肝气逆证 海马 γ-氨基丁酸A受体β2亚基 半定量RT-PCR 大鼠 

分 类 号：R241.6[医药卫生—中医诊断学]