气相色谱法测定苦参及其制剂中的苦参碱类生物碱  

Determination of Matrine Type in Sophora Flavescens Ait. and Its Preparations by Gas Chromatography

作　　者：江林 王海霞 

机构地区：[1]地矿部岩矿测试技术研究所

出　　处：《分析测试仪器通讯》1997年第4期222-227,共6页

摘　　要：建立了填充柱气相色谱测定苦参及其制剂中的苦参碱类生物碱的方法。色谱柱为１ｍ长的ＯＶ＿１７填充柱，检测器及汽化室温度为３２０℃，升温方式为：１５０℃→２５０℃，２５０℃保持３ｍｉｎ，２５０℃→３００℃，３００℃保持２ｍｉｎ，升温速度均为２０℃／ｍｉｎ。该方法对由数种中草药（含苦参）组成的醇提取液有满意的精密度（ＲＳＤ＝１．９％，ｎ＝１１）及回收率（９８．３％）。通过对苦参碱、氧化苦参碱的填充柱色谱行为与毛细管柱色谱＿质谱行为对比，得知填充柱色谱峰为苦参总碱的峰。A method for the determination of matrine type in sophora flavescens ait. and its preparations by gas chromatograph with packed column has been developed. The column with a length of 1 meter is packed with OV_17. The temperature of detector (FID) and injector is 320℃.The colu￣mn temperature is programmed from 150 ℃to 250℃ at a rate of 20℃/min, holding 3 minutes at 250℃, and then from 250℃to 300℃ at a rate of 20℃/min.,holding 2 minutes at 300℃.This method has been applied to determine matrine in samples which are extracted from several kinds of Chinese medicine including sophara flavescens ait. with satisfactory of precision(RSD=1.9%, n =11) and recovery(98.3%). By comparison the behavior of matrine and oxymatrine in gas chromatograph with packed column and in gas chromatograph/mass spectrograph with capillary column, a conclusion was made that the peak in packed column is proved to be total matrine type.

关 键 词：气相色谱法 苦参 苦参碱类生物碱 苦参制剂 

分 类 号：R282.710.5[医药卫生—中药学] R284.1[医药卫生—中医学]