香蕉果实的1个14-3-3蛋白同源基因的克隆及序列分析(简报)  

Isolation and Characterization of a Homolog Encoding 14-3-3 Protein from Banana Fruit

作　　者：李美英[1] 徐碧玉[1] 刘菊华[1] 杨小亮[1] 金志强[1] 

机构地区：[1]中国热带农业科学院热带生物技术研究所

出　　处：《热带作物学报》2008年第4期415-418,共4页Chinese Journal of Tropical Crops

基　　金：国家自然科学基金资助项目(编号:30471207);公益性行业(农业)科研专项(编号:nyhyzx07-029)

摘　　要：根据在香蕉果实抑制缩减文库中获得的1个14-3-3蛋白基因片段,采用PCR和RACE相结合的方法从香蕉(Musaacuminate L. AAA group cv. Brazilian)cDNA文库中筛选到其全长cDNA序列。序列测定和Blastx比对分析结果表明,该cDNA全长1 037 bp,含有1个完整的阅读框,其编码区编码261个氨基酸残基,具有植物14-3-3蛋白基因的保守结构域,并与其他植物来源的14-3-3蛋白具有很高的同源性,将其命名为Ma-14-3-3b(Musa acuminate 14-3-3)。采用遗传进化系统发育树的分析结果表明,Ma-14-3-3b与来源于单子叶植物的多数14-3-3蛋白基因序列在同一个进化枝上。采用RT-PCR对其在香蕉果实发育不同时期的表达进行分析结果表明,在香蕉果实发育的不同时期差异表达,推测其可能在果实的发育中起作用。A cDNA fragment homologous with 14-3-3 gene was obtained in the cDNA library of banana （Musa acuminata L. AAA group cv. Brazilian） constructed by SSH, based on which a full length of the eDNA was obtained by using PCR and RACE with cDNA library of banana fruit as templates. The full length of this cDNA was 1 037 bp with an open reading frame encoding 261 amino acids. Alignment of deduced amino acid sequence with those from other plants revealed that the eDNA shared high homology with 14-3-3 protein genes from other plants, and was designated as Musa acuminata 14-3-3 gene （Ma-14-3-3b）. Phylogenetic analysis revealed that Ma-14-3-3b had closer genetic relationship with those from monocotyledon species than those from other species. This indicates Ma-14-3-3b belongs to the same lineage of 14-3-3 from monocotyledon. RT-PCR was used to analyze expression patterns of Ma-14-3-3b in different stages of banana fruit growth and development. The results showed that Ma-14-3-3b was expressed differentially at different stages of growth and development of banana fruit, which suggests that Ma-14-3-3b play roles in growth and development of banana fruit.

关 键 词：香蕉(Musancuminate L.AAA group cv.Brazilian) Ma-14—3—3b 14—3—3蛋白 

分 类 号：S668.101[农业科学—果树学]