海南胡椒中黄瓜花叶病毒分离物的分子鉴定  

Molecular Diagnosis of Cucumber Mosaic Virus from Piper nigrum Linn. in Hainan

作　　者：牛立霞[1] 王健华[1] 冯团诚[1] 刘志昕[1] 

机构地区：[1]中国热带农业科学院热带生物技术研究所,海口571101

出　　处：《热带作物学报》2008年第4期510-513,共4页Chinese Journal of Tropical Crops

基　　金：国家科技支撑计划课题(2007BAD48B01);中央级科研院所基本科研业务资助

摘　　要：通过间接酶联免疫法,从海南罹病胡椒植株中检测到黄瓜花叶病毒(Cucumber mosaic virus,CMV)。从病叶中提取总RNA,用巢式RT-PCR方法扩增获得657 bp的CMV CP基因片段,并与pMD18-T载体连接和转入大肠杆菌克隆,然后进行测序。序列分析结果表明,该分离物与CMV亚组Ⅰ、亚组Ⅱ之间的核苷酸序列同源性分别为92.54%～99.09%和76.97%～77.42%,推导氨基酸序列同源性分别为98.17%～100%和81.74%～82.65%。该分离物CP基因与印度胡椒分离物同源性相对较低,应属不同株系。Cucumber mosaic virus was detected from infected Piper nigrum Linn. in Hainan by using the indirect enzyme-linked immunosorbent assay. Total RNA was extracted from infected leaves and the cDNA fragment of the coat protein （CP） gene obtained by nested RT-PCR was 657 bp in length. The amplified CP fragment was linked with pMD 18-T vector, cloned in E.coli cells, and then sequenced. The sequence analysis between the CMV-China CP fragment and those of the subgroup I and subgroup II shows that the CMV-China CP shares 92.54 %-99.09 % and 76.97 %-77.42 % nucleotide sequence identity and 98.17 %-100% and 81.74 %-82.65 % deduced amino acids identity, respectively, and that homology between CMV-China and CMV isolated from Piper nigrum Linn. in India is relatively low by comparison. This suggests that CMV-China isolate fall within the CMV subgroup I.

关 键 词：胡椒 黄瓜花叶病毒 血清学 CP基因 序列分析 

分 类 号：S435.73[农业科学—农业昆虫与害虫防治]