贵州省八个种群角倍蚜ISSR遗传多样性  

Genetic diversity of eight Schlechtendalia chinensis populations from Guizhou Province:A study with inter-simple sequence repeats markers

作　　者：王定江 杨汉远 钟扬[2] 任竹梅[3] 

机构地区：[1]贵州省黔东南州林业科学研究所,贵州凯里556000 [2]复旦大学生命科学学院,上海200433 [3]山西大学生命科学与技术学院,太原030006

出　　处：《生态学杂志》2008年第10期1729-1733,共5页Chinese Journal of Ecology

基　　金：国家自然科学基金项目(30670361);山西省自然科学基金资助项目(2007011078)

摘　　要：基于ISSR分子标记技术对采自贵州省8个种群共139个角倍蚜样本进行遗传多样性及其与环境因子的相关性分析。筛选的12条引物共扩增出多态性条带133条，没有种群特异带出现，平均每个引物扩增的多态带数为11条，Popgene软件分析显示，总群体多态性条带比率为100％，尽管不是特意选择变异高的引物。种群间遗传相似系数变异范围介于0．899～0．955，遗传距离介于0．046～0．106，说明角倍蚜不同种群之间的遗传分化不大。聚类分析显示，8个种群共形成2个大的聚类簇，各种群之间没有明显的地域性分布规律，但遗传多样性与3月温度和3月降水量呈负相关。Inter-simple sequence repeat （ISSR） molecular markers were used to detect the genetic diversity among 139 individuals from 8 populations of Chinese sumac aphid Schlechtendalia chinensis collected from Guizhou Province, China. 22 ISSR primers were evaluated for their ability to produce polymorphic bands, and these primers yielded a total of 133 scorable.bands, averaged 11 bands each primer. There was no population-specific marker, which was present in one population but absent in the others. Popgene polymorphic, even though the primers were not analysis indicated that 200% of these loci were intentionally selected for high variability. The genetic similarity was 0. 899-0. 955, and the genetic distance was 0. 046-0. 106, indicating a lower genetic diversity of S. chinensis populations. Cluster analyses showed that the aphid populations were divided into 2 groups, and the genetic diversity had no evident correlation with geographic area but negatively correlated with the mean air temperature and rainfall in March.

关 键 词：角倍蚜 资源昆虫 ISSR 遗传多样性 环境因子 

分 类 号：Q96[生物学—昆虫学]