核糖核酸干扰技术研究进展  

RNAi Technique and Its Research Progress

作　　者：方华舟[1] 涂知明[2] 

机构地区：[1]荆楚理工学院生物工程学院,湖北荆门448000 [2]华中科技大学生命科学与技术学院,武汉430074

出　　处：《湖北农业科学》2008年第9期1088-1091,共4页Hubei Agricultural Sciences

基　　金：湖北省科技攻关项目(2005AA201C53)

摘　　要：核糖核酸干扰技术(RNAi)是遗传工程领域近几年来取得的重大技术进展之一,其理论体系及技术规范已基本形成。RNAi是一种高效、特异性强的基因阻断技术,被誉为将在功能基因组学及遗传工程领域掀起一场真正的革命。介绍了RNAi的发现、发展历程,阐述了RNAi的作用机理及技术特点,探讨了小分子干扰RNA导入细胞的技术策略以及对RNAi进行深入研究的重大意义。RNAi technique was one of the hot topics in genetic engineering area and developed very quickly. Its theory and teehnique system had already formed. It was a highly efficient and specific gene knockout method to make target gene silent, and it would bring a revolution in genomics and genetic engineering. This article introduced the discovery and development history of RNAi, explained the mechanism of RNAi, concluded the characteristies of RNAi techniques, and explored the strategy to transform the SiRNA to target cell and the meaning of RNAi intensive research.

关 键 词：核糖核酸干扰技术(RNAi) 作用机理 应用 

分 类 号：Q522[生物学—生物化学] Q752