CpG ODN联合STAg免疫小鼠诱导黏膜IgA免疫应答  

Mucosal immune responses after intranasal immunizeation of CpG ODN plus Toxoplasma gondii STAg in BALB/c mice

作　　者：石荣[1] 殷国荣[1] 孟晓丽[1] 刘娟娟[1] 刘红丽[1] 王海龙[1] 

机构地区：[1]山西医科大学医学寄生虫学研究所寄生虫学教研室,太原030001

出　　处：《中国公共卫生》2008年第10期1197-1199,共3页Chinese Journal of Public Health

基　　金：国家自然科学基金(30640057);山西省自然科学基金(20041105);山西省高校科技研究开发重点项目(20041238);山西省青年科技基金(20051045)

摘　　要：目的观察GpG寡核苷酸（CpG ODN）联合刚地弓形虫可溶性速殖子抗原（soluble tachyzoite antigen,STAg）滴鼻免疫BALB/c小鼠诱异的不同黏膜部位IgA免疫应答动态变化,探讨CpG ODN作为弓形虫复合黏膜疫苗的佐剂效应。方法雌性BALB/c小鼠72只,随机分为实验组和对照组,每组36只。实验组以10μg CpG ODN联合20μg STAg滴鼻免疫,对照组以磷酸盐缓冲液（PBS）滴鼻。间隔2周,免疫2次,于末次免疫后第1,2,3,4,5,6周分别随机处死小鼠（6只/组）。ELISA法测定鼻咽冲洗液、肺冲洗液、小肠冲洗液、阴道冲洗液IgA。结果实验组小鼠各部冲洗液中IgA平均水平高于对照组。鼻咽冲洗液中IgA水平在第1周时最高,各时间点虽高于对照组,但差异无统计学意义。肺冲洗液IgA在第5周达到高峰,第4,5,6周高于对照组（P〈0.001）。小肠冲洗液中弓形虫特异性IgA抗体水平在各个时间点均高于对照组,第2,3周（P〈0.001）,4周（P〈0.05）差异有统计学意义。阴道冲洗液IgA在免疫后第1-6周显著高于对照组（P〈0.01）,第5周时水平最高。结论CpG ODN作为STAg的佐剂滴鼻免疫BALB/c小鼠可诱异黏膜部位产生高水平IgA抗体,且可持续较长时间。Objective To investigate the development of different mucosal site immune responses induced by intransal immunization with CpG ODN plus Toxoplasrna gondii soluble tachyzoite antigen（STAg） and the adjuvant effect of CpG ODN acting as complex mucosal vaccine adjuvant against Toxoplasrna gondii. Methods Seventytwo 5 - to- 6 week - old female BALB/c mice were divided into control group and infected group. The mice of infected group were immunized intranasally two times at 14 - day intervals with 10μg CpG ODN plus 20μg STAg. The mice of control group were immunized intranasatly with PBS at the same time. Six mice in each group were killed by dislocation of cervical vertebra on week 1,2, 3, 4, 5, 6 respectively after last immunity. IgA in rinse solution of nasopharynx, lung, intestinal, and vaginal were detected by ELISA. Results The average IgA level in each of the infected group was higher than that of the control group. IgA in nasopharynx wash samles was the highest on week 1 and it was higher than that of the control on each week but the difference was not significant. IgA in lung wash samples achieved the peak value on the 5th week and was higher than that of the control on week 4, 5.6（ P 〈 0. 001 ）. IgA in intestine wash samples was higher than that of the control in each week and increased significantly on week 2, 3（ P 〈 0.01 ）and the level was the highest on week 5. Conclusion Intranasal immunization with CpG ODN adjuvant and STAg can effectively induce persistent mucosal immune responses.

关 键 词：刚地弓形虫 速殖子抗原(STAg) CpG寡核苷酸(CpGODN) 黏膜免疫 黏膜佐剂 IgA 

分 类 号：R392[医药卫生—免疫学]