三羟基苯甲酸对淋巴瘤细胞凋亡及周期影响  

Effect of TAD compound on apoptosis and cell cycle progression of lymphoma cells in vitro

作　　者：吕喆[1] 龚守良[1] 牛凤兰[2] 李鹏武[1] 李晨旭[2] 

机构地区：[1]吉林大学公共卫生学院卫生部放射生物重点实验室,长春130021 [2]吉林大学公共卫生学院卫生检测教研室作

出　　处：《中国公共卫生》2008年第10期1210-1211,共2页Chinese Journal of Public Health

基　　金：吉林省科技厅重点资助项目(20070424)

摘　　要：目的观察不同剂量3,4,5三羟基苯甲酸化合物（TAD）对小鼠淋巴瘤细胞株EL-4细胞的存活率、细胞凋亡及细胞周期进程的影响,探讨其对肿瘤生长抑制的机制。方法应用四甲基偶氮噻唑蓝（MTT）比色法和流式细胞仪检测经不同浓度（3.125-25μg/ml）TAD处理的EL-4细胞存活率、细胞凋亡率及细胞周期的变化。结果EL-4细胞经过3.125,6.25,12.5和25μg/ml TAD处理后,各观察组细胞存活率依次为（80.60±6.01）%,（75.39±4.97）%,（71.42±3.73）%和（54.96±4.57）%;与对照组100%存活率相比明显降低（P〈0.01,P〈0.001）。细胞凋亡率依次为（4.95±1.76）%,（19.58±7.91）%,（35.85±7.01）%,（11.67±2.96）%;明显高于对照组的（0.36±0.33）%（P〈0.01,P〈0.001）。TAD引起细胞周期进程的改变,G1期细胞百分数明显高于对照组（P〈0.05,P〈0.01,P〈0.001）,S期细胞百分数明显低于对照组（P〈0.05,P〈0.01,P〈0.001）;G2期细胞无明显变化。结论TAD能够降低EL-4细胞存活率,诱导EL-4细胞凋亡,阻止G1期细胞向S期进程,其抑制作用可能通过G0/G1期阻滞引起。Objective To observe the effect of 3, 4, 5 - trihydroxybenzoic acid dimmer （TAD） in different concentration on apoptosis and cell cycle progression of lyrnphoma EL - 4 cells in vitro and explore its possible mechanism of tumor growth inhibition. Methods Survival rate was observed with MTT assay. The changes of apoptosis and cell cycle progression were measured with flow cytometry. Results TAD of 3. 125, 6.25, 12.5 and 25 μg/rrd could inhibit the pro/ifemtion of E1 - 4 lymphoma cells in vitro. The survival rates of the cells treated with TAD were significantly lower than that of in the control （P〈0.01, P〈 0.001 ）. The apoptotic percentages of the cells treated with TAD increased signficantly as compared with those in the control（P 〈 0.01, P 〈 0. 001 ）. And the percentages of G0/G1 phase cells treated with TAD were significantly higher than those of in the control （P 〈 0.05, P 〈 0.01, P 〈 0. 001 ）. While the percentages of S phase cells treated with TAD were significantly lower than those in the control（P 〈 0.05, P 〈 0.01, P 〈 0. 001 ）. The percentages of G2 phase cells did not change significantly. Conclusion The inhibiting effect of TAD on tumor growth could be due to cell apoptosis caused by G0/G1 phase arrest.

关 键 词：三羟基苯甲酸 EL-4细胞 存活率 细胞凋亡 细胞周期 

分 类 号：R965[医药卫生—药理学] R733[医药卫生—药学]