基因转染树突状细胞联合免疫抗血吸虫感染作用  

Study on immune effect of Sj26 and Sj23 gene transfered dendritic cells against Schistosoma japonicum

作　　者：沈定文[1] 罗金萍[1] 李雍龙[2] 

机构地区：[1]湖北咸宁学院医学院寄生虫学教研室,咸宁437100 [2]华中科技大学同济医学院

出　　处：《中国公共卫生》2008年第10期1216-1217,共2页Chinese Journal of Public Health

基　　金：湖北省自然科学基金(2007ABA191);湖北省教育厅项目(D200728001)

摘　　要：目的探讨日本血吸虫Sj26和Sj23基因转染树突状细胞(DC)联合免疫抗血吸虫感染保护性免疫作用。方法利用Sj26-Sj23基因转染、Sj26基因转染、Sj23基因转染、空质粒pcDNA3转染和未处理DC分别免疫BALB/c小鼠3次,攻击感染后计数成虫和虫卵。结果Sj26-Sj23基因转染DC联合免疫小鼠诱导40.5%的减虫率和58.9%和减卵率,高于单独Sj26基因转染DC免疫组(34.5%和48.5%)和Sj23基因转染DC免疫组(32.6%和40.6%),亦明显高于空质粒pcDNA3转染DC组(16.8%和15.8%)和未处理DC组(14.6%和14.4%),Sj26基因转染DC免疫组和Sj23基因转染DC免染组的减卵率有差异有统计学意义。结论Sj26-Sj23基因转染DC联合免疫可增强抗血吸虫感染的保护性免疫作用。Objective To explore the effect of Sj26 and Sj23 gene transfered dendritic cells（DC） against the irffection of Schistosoma japonicum. Methods BALB/c mice were immumized three times with Sj26 and Sj23 gene transfered DC, Sj26 gene transfered DC, Sj23 gene trartsfered DC, plasmid pcDNA3 transfered DC and untreated DC, respectively. The number of adult worm and the egg number in the liver were calculated six weeks after challenging with the cercariae of Schistosoma japonlcum. Results The rate of worm reduction and egg reduction in the group of Sj26 and Sj23 gene transfered DC was 40.5% and 58.9% respectively, higher than that of the group Of Sj26 gene transfered DC（34.5% and 48.5% ）and Sj23 gene transfered DC （32.6 % and 40.6 % ）, also significandy higher than that of group of plasmid pcDNA3 transfered DC （16.8 % and 15.8 % ） and untreated DC （ 14.6 % and 14.4 % ）. There was significant difference of egg reduction rate between group of $j26 transfered DC and group of Sj23 transfered DC. Conclusion The results indicate that Sj26 gene transfered DG can induce and enhance protective immunity against the infection of Schistosoma japonicum.

关 键 词：日本血吸虫 树突状细胞 SJ26 SJ23 基因转染 保护性免疫 

分 类 号：R383.2[医药卫生—医学寄生虫学]