X射线对Jurkat和EL-4细胞周期进程的影响  

Effects of X-rays on cell cycle progression of Jurkat and EL-4 cell lines

作　　者：武宁[1] 王珍琦[1] 李鹏武[1] 吕杰[1] 刘继顺[1] 张萱[1] 

机构地区：[1]吉林大学公共卫生学院卫生部放射生物学重点实验室,吉林长春130021

出　　处：《吉林大学学报（医学版）》2008年第5期729-732,共4页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金资助课题(20675079)

摘　　要：目的:探讨较大剂量X射线照射对Jurkat和EL-42种T淋巴细胞周期进程的影响。方法:采用碘化丙啶(PI)荧光标记及流式细胞术(FCM)分别检测2.0GyX射线照射后不同时间点(0、4、8、16、24和48h)Jurkat与EL-4细胞周期进程变化的时间-效应关系和1.0、2.0和4.0Gy不同剂量X射线照射后两者的剂量-效应关系。结果:与各时间点对应的假照组比较,2.0GyX射线照射后Jurkat细胞G0/G1期和S期细胞百分率降低(P<0.01),G2+M期细胞百分率增高(P<0.01),于照射后24h变化最为显著;EL-4细胞G2+M期细胞百分率于照射后4～8h增高(P<0.01),G0/G1期细胞百分率于照射后8～48h增高(P<0.05或P<0.01),S期细胞百分率于照射后8～24h降低(P<0.01)。1.0～4.0GyX射线照射后16h,随着剂量的增加,与假照组比较,Jurkat细胞G0/G1期和S期细胞百分率降低(P<0.05或P<0.01),G2+M期细胞百分率增加(P<0.01);随着剂量的增加,与假照组比较,EL-4细胞G0/G1期细胞百分率升高(P<0.01),S期细胞百分率降低(P<0.01),G2+M期细胞百分率仅在4.0GyX射线照射后显著增高(P<0.01)。结论:1.0～4.0GyX射线照射可以改变Jurkat与EL-4细胞周期进程,诱导Jurkat细胞发生G2期阻滞,EL-4细胞发生G1期和G2期阻滞。Objective To investigate the effect of X-irradiation on cell cycle progression of Jurkat and EL-4 cell lines. Methods The time- and dose-effects of cell cycle progression in Jurkat and EL-4 lymphoma cell lines after X- irradiation with different doses （1.0, 2.0 and 4. 0 Gy） were analyzed by flow cytometry （FCM） following staining with propidium iodide （PI）. Results In time-effect experiment; it was demonstrated that the percentages of Jurkat cells in G0/G1 and S phases were decreased significantly after 2.0 Gy X-irradiation （P〈0.01）, whereas G2 ＋ M phases cells were increased significantly at the same time compared with sham-irradiation （P〈0.01）. It was also showed that the percentages of EL-4 cells in G2＋M and G0/G1 phases were increased （P〈0. 05, P〈0. 01）, and S phase cells were decreased significantly （P〈0.01） . For dose-effect experiment, the results showed that the percentages of Jurkat cells in G0/G1 and S phases were decreased significantly 16 h after X-irradiation with the doses of 1.0, 2. 0 and 4.0 Gy （P〈0.05, P〈0.01）, and G2 ＋M phases cells were increased compared with sham irradiation （P〈0.01） . The percentage of EL-4 cells in G0/G1 phase was increased （P〈0.01）, and S phase cells were decreased （P〈0.01）. However, the percentage of EL-4 cells in G2 ＋ M phases was increased significantly only after 4. 0 Gy X-irradiation （P〈0.01）. Conclusion The cell cycle progression of Jurkat and EL-4 lymphoma cell lines could be changed by X-irradiation with the doses of 1.0, 2. 0 and 4.0 Gy. X-irradiation （1.0, 2.0 and 4.0 Gy ） could induce G2 arrest in Jurkat cells, G2 and G1 arrest in EL-4 ceils.

关 键 词：X射线 人T淋巴细胞白血病细胞株 小鼠T淋巴瘤细胞株 细胞周期 

分 类 号：R817.1[医药卫生—影像医学与核医学]