松茸多糖对X射线照射小鼠造血功能的保护作用  

Protective effect of polysaccharides of tricholoma matsutake on hematopoietic function of X-rays irradiated mice

作　　者：王宏芳[1] 林晓晨[2] 李雪静[1] 李静[1] 徐坤[1] 宋秀玲[1] 李娟[1] 

机构地区：[1]吉林大学公共卫生学院卫生检验教研室,吉林长春130021 [2]吉林大学畜牧兽医学院

出　　处：《吉林大学学报（医学版）》2008年第5期751-754,共4页Journal of Jilin University：Medicine Edition

基　　金：国家重点基础研究发展计划(973计划)资助课题(2007CB808000)

摘　　要：目的:探讨松茸多糖(PTM)对辐射损伤小鼠造血功能的保护作用。方法:将50只ICR小鼠随机分成5组,每组10只。正常对照组(NC)和辐射对照组(IC)给予生理盐水灌胃,3个剂量PTM组分别给予400、800和1200mg.kg-1剂量PTM灌胃,每天1次,连续7d。第8天给予IC组和PTM组动物进行全身一次性照射,总剂量2.0Gy,检测小鼠造血干细胞集落形成单位(CFU-S)数、骨髓基质细胞-成纤维祖细胞集落形成单位(CFU-F)数、骨髓细胞周期和骨髓微核率。结果:与IC组比较,预防给予PTM各组小鼠CFU-S数量明显减少(P<0.05或P<0.01)、骨髓CFU-F数显著升高(P<0.05或P<0.01),中、高剂量PTM组PCE微核的数量明显降低(P<0.05或P<0.01),G1期细胞数明显减少(P<0.05或P<0.01),S期细胞数明显增加(P<0.05或P<0.01)。结论:松茸多糖对辐射所致的造血损伤有明显的保护作用。Objective To explore the protective effect of the polysaccharides of tricholoma matsutake （PTM） on hematopoietic function of X-rays irradiated mice. Methods Fifty ICR mice were divided into five groups randomly, 10 each group. The mice in normal control group （NC） and radiation control group （IC） were gaster-poured by 0.9 % sodium chloride solution. The mice in PTM groups were gaster-poured by three different doses PTM （400, 800, 1200 mg·kg^-1） for 7 d. The mice all groups except NC group were irradiated once with 2.0 Gy X-rays on the eighth day. The conony forming units-spleen （CFU-S）, fibrolast colony forming unit （CFU-F）, cell cycle of bone marrow cells and micronucleus rate of bone marrow polychromalic erythrocytes （PCE） were detected after radiation for 24 h. Results Compared with IC group, the number of CFU-S in PTM groups decreased significantly （P〈0.05 or P〈0.01） and the number of CFU-F increased markedly （P〈0.05 or P〈0.01）; in high and moderate doses PTM the micronucleus rates of bone marrow PCE and the percentage of bone marrow cells at G1 phase decreased signficantly （ P〈0.05 or P〈0.01）; the percentage of cells at S phase increased signficantly （ P〈0.05 or P〈0.01）. Conclusion PTM has an obvious protective effect on hematopoietic function of X-rays irradiated mice.

关 键 词：松茸多糖 辐射防护 造血系统 细胞周期 

分 类 号：R285[医药卫生—中药学]