银耳孢糖对白细胞减少小鼠的升白细胞作用  

Leukogenic effect of tremella fuciformis spores polysaccharide on leukopenia mice

作　　者：徐华丽[1] 于晓风[1] 曲绍春[1] 张锐[1] 王迎庆[1] 睢大员[1] 

机构地区：[1]吉林大学药学院药理学教研室,吉林长春130021

出　　处：《吉林大学学报（医学版）》2008年第5期759-762,共4页Journal of Jilin University：Medicine Edition

基　　金：国家九五科技攻关项目资助课题(96-901-06-68);吉林省科技厅科技发展计划重点项目资助课题(20010443)

摘　　要：目的:观察银耳孢糖(TSP)对环磷酰胺(CTX)和X射线所致白细胞减少小鼠的升高白细胞作用。方法:60只昆明种小鼠随机分为6组,每组10只:正常对照组、模型对照组、阳性对照组和TSP小、中和大剂量组。正常对照组未施加任何处理,其他5组腹腔注射CTX100mg.kg-1或X射线照射,造成白细胞减少模型。正常对照组及模型对照组尾静脉注射氯化钠注射液,阳性药物组尾静脉注射苦参素注射液200mg.kg-1,药物组分别尾静脉注射TSP25、50和100mg.kg-1,共15d;各组小鼠分别于第1次给予CTX或X射线照射前和给予CTX或X射线照射后3、6、9、12和15d计数外周血白细胞数,取右侧完整股骨检查骨髓有核细胞数。结果:给予CTX或X射线照射后,各组白细胞数和骨髓有核细胞与正常对照组比较均明显下降(P<0.05),与模型组比较,TSP50、100mg.kg-1组白细胞和骨髓有核细胞数升高(P<0.05或P<0.01)。结论:TSP对CTX或X射线照射小鼠外周血白细胞减少具有明显的升白细胞作用。Objective To observe the leukogenic effect of tremella fuciformis spores polysaccharide （TSP） on leukopenia mice induced by cyclphohamide （CTX） and X-rays. Mice were randomly divided into six groups： normal control group （untreated normal animals）; negative control group; positive control group; small, middle, large doses TSP groups. The mice of each group （except normal control group） were administered with CTX （100 mg·kg^-1） intraperitoneally or exposed to X-rays to result in leucopenia. In the normal and negative control groups, each animal was administered with saline; in TSP groups, mice were administered with TSP 25, 50, 100 mg·kg^-1 through caudal vein every day for 15 d; in positive control group, each animal was administered with matrine （200 mg·kg^-1） through caudal vein every day for 15 d. The numbers of peripheral leukocytes of all animals were counted before CTX treatment or exposure to X-rays and 3, 6, 9, 12 and 15 d after treatment. The number of bone marrow karyotes of the mice was examined. Results Compared with normal control group, the leucocytes and the number of bone marrow karyotes of mice were remarkably decreased in the other groups after treated with CTX or exposure to X-rays （P〈0.05）, hut leucocytes and the number of bone marrow karyotes were increased in TSP 50 and 100 mg·kg^-1 groups compared with negative control group （P〈0.05）. Conclusion TSP has the effect of the leukoeytopoiesis promoting in the leukopenia mice induced by CTX and X-rays.

关 键 词：银耳孢糖 白细胞生成 环磷酰胺 X射线 生物反应调节剂 

分 类 号：R285.5[医药卫生—中药学] R979.1[医药卫生—中医学]